tailieunhanh - An electrophoretic study on esterase and blood serum proteins of the water vole, Arvicola terrestris (L., 1758) (Mammalia: Rodentia), in Kirflehir province

Esterase enzymes from 30 specimens of Arvicola terrestris in 4 localities in Kirflehir province were examined using starch gel electrophoresis and blood serum proteins from 27 specimens using SDS-PAGE. It was determined that the esterase enzyme had 2 alleles (A and B), which were coded by 1 locus. A and B allele frequencies were and , respectively. | Turk J Biol 28 (2004) 47-53 © TÜB‹TAK An Electrophoretic Study on Esterase and Blood Serum Proteins of the Water Vole, Arvicola terrestris (L., 1758) (Mammalia: Rodentia), in K›rflehir Province* Ceren ‹Y‹GÜN, Reyhan ÇOLAK Department of Biology, Faculty of Science, Ankara University, Ankara - TURKEY Received: Abstract: Esterase enzymes from 30 specimens of Arvicola terrestris in 4 localities in K›rflehir province were examined using starch gel electrophoresis and blood serum proteins from 27 specimens using SDS-PAGE. It was determined that the esterase enzyme had 2 alleles (A and B), which were coded by 1 locus. A and B allele frequencies were and , respectively. The heterozygosity level was . It was determined that there were variations in the globulin, prealbumin and postalbumin regions, while the albumin region was monomorphic. There were 6-13 bands in the globulin region, 1-2 bands in the postalbumin zone and 2-4 bands in the prealbumin zone. Key Words: Arvicola terrestris, esterase, serum proteins, electrophoresis, K›rflehir K›rflehir Yöresindeki Su S›çan› Arvicola terrestris (L., 1758) (Mammalia: Rodentia)’in Esteraz ve Kan Serum Proteinleri Üzerine Elekroforetik Bir Çal›flma Özet: K›rflehir yöresinden dört lokaliteden Arvicola terrestris’e ait 30 örne¤in esteraz enzimi niflasta jel elektroforez tekni¤i ve 27 örne¤in kan serum proteinleri SDS-PAGE (sodium dodecyl sulphate - polyacrylamide gel electrophoresis) tekni¤i ile incelendi. Esteraz enziminin bir lokus taraf›ndan kodlanan iki alele (A ve B) sahip oldu¤u belirlendi. A alelinin frekans› , B alelinin frekans› ise olarak belirlendi. Bu lokusta heterozigotluk oran› olarak hesapland›. Albumin bölgesi monomorfik iken globülin, postalbümin ve prealbumin bölgelerinde varyasyon belirlendi. Globülin bölgesinde 6-13 bant, postalbümin zonunda 1-2 bant ve prealbümin zonunda 2-4 bant belirlendi. Anahtar Sözcükler: Arvicola terrestris, esteraz, serum proteinleri, .