tailieunhanh - Adventitious shoot organogenesis and plant regeneration from cotyledons of diploid Diyarbakir watermelon (Citrullus lanatus cv. “Sürme”)

Adventitious shoots were obtained from the diploid Diyarbakir watermelon cultivar "Sürme" by culturing cotyledons on shoot regeneration medium for 3 weeks. The effects of two cytokinins, benzyl amino purine (BA) and kinetine (Kin), on shoot organogenesis were examined. The number of shoots per explant were approximately more than 50% higher when mg/l BA was used compared to the most effective kinetin concentration (1 mg/l). | Turk J Biol 27 (2003) 101-105 © TÜB‹TAK Adventitious Shoot Organogenesis and Plant Regeneration from Cotyledons of Diploid Diyarbak›r Watermelon (Citrullus lanatus cv. “Sürme”) Vedat P‹R‹NÇ* Department of Horticulture, Faculty of Agriculture, Dicle University, 21280 Diyarbak›r - TURKEY Ahmet ONAY Department of Biology Faculty of Science and Literature, Dicle University, 21280 Diyarbak›r - TURKEY Hakan YILDIRIM Department of Horticulture, Faculty of Agriculture, Dicle University, 21280 Diyarbak›r - TURKEY Filiz ADIYAMAN, Çi¤dem IfiIKALAN, Davut BAfiARAN Department of Biology Faculty of Science and Literature, Dicle University, 21280 Diyarbak›r - TURKEY Received: Abstract: Adventitious shoots were obtained from the diploid Diyarbak›r watermelon cultivar "Sürme" by culturing cotyledons on shoot regeneration medium for 3 weeks. The effects of two cytokinins, benzyl amino purine (BA) and kinetine (Kin), on shoot organogenesis were examined. The number of shoots per explant were approximately more than 50% higher when mg/l BA was used compared to the most effective kinetin concentration (1 mg/l). Plants were obtained by transferring 1 to 2 cm shoots to MS medium supplemented with 1-4 mg/l naphthalene acetic acid. Most of the regenerated plants (more than 50%) were successfully transplanted to the field. This demonstrates that in vitro propagation can be used to produce high quality diploid Diyarbak›r watermelon for use in breeding lines. Key Words: Regeneration, Tissue culture, Watermelon Diploid Diyarbak›r Karpuzu (Citrullus lanatus cv. "Sürme") Kotiledonlar›ndan Adventif Sürgün Organogenezisi ve Bitki Rejenerasyonu Özet: Diploid Diyarbak›r karpuzunun (Citrullus lanatus cv. "Sürme’’) kotiledonlar›ndan 3 haftal›k kültür sonucunda adventif sürgünler oluflturuldu. Sürgün organojenezisi üzerine iki tip sitokininin; benzil aminopürin (BA) ve kinetin (Kin) farkl› konsantrasyonlar›n›n etkileri araflt›r›ld›. Eksplant bafl›na oluflan sürgün say›s› .

• Sinh học
• Tissue culture
• Shoot organogenesis
• Plant regeneration
• Diyarbakir watermelon
• Naphthalene acetic acid
• Advances in tissue culture
• Tissue culture of cucurbits
• Strategies for tissue culture
• Propagation in cucurbits
• plant tissue culture
• plant tissue culture practice
• tissue culture technology
• tissue engineering applications
• plant tissue
• microorganisms
• introduction to animal cell culture
• Types of tissue culture
• Cell culture
• Primary explant culture
• Organ culture
• Plant tissue and cell culture
• Cell culture in Vietnam
• Forty five years of development
• Plant tissue in Vietnam
• Plant Propagation
• The Background
• Plant Tissue Culture Procedure
• Developmental Biology
• Somatic Embryogenesis
• Clonal progagation of Vietnamese
• Orthosiphon stamineus benth
• Sinensetin using plant tissue culture technique
• Plant tissue culture technique
• Accession containing high content
• Tissue culture embryo
• Mature embryos
• Evaluation and validation of rice
• Rice (Oryza sativa L
• Genotypes for tissue culture response
• Pharmaceutical compounds
• Secondary metabolism
• Value pharmaceutical compounds
• Plant tissue culture technology
• Secondary hardened
• Tissue culture plantlets
• Secondary hardened tissue culture plantlets
• Growth and yield of banana
• Banana as influenced by age
• Low cost medium
• MS medium
• Cost effectiveness
• Conventional tissue culture media
• Low cost alternatives
• Hydrangea macrophylla
• In vitro
• Large scales of hydrangea macrophylla
• Tissue culture technique
• Hydrangea macrophylla using tissue culture technique
• Plant density
• Ney pooovan
• Tissue culture banana
• Growth tissue culture banana
• planting density on growth
• Advanced materials
• Advanced devices
• Co culture systems
• Cell culture insert
• Clip on scaffold
• Tissue engineering
• Tissue transportation
• Cherry rootstock rooting
• Amino acids
• Biochemical characteristics
• Endangered plant
• Endangered plant species
• Technique for propagation
• Review on plant tissue culture
• Micro propagation
• Potato (Solanum tuberosum L )
• Tissue culture of potato
• In vitro micro propagation
• Culture medium
• Prunus dulcis
• Tissue culture medium
• Nas hypothesis
• Micropropagation medium
• Indole 3 butyric acid
• Culture Media
• Emerging Economy
• Shoot Regeneration
• Abiotic Stress Conditions
• BMC Biotechnology
• Panicum hallii
• Panicum virgatum
• Suspension culture
• BMC Musculoskeletal Disorders
• Periprosthetic joint infection
• Periprosthetic tissue
• Blood culture bottles
• Meta analysis
• Diagnostic performance
• Kappaphycus alvarezii
• Callus induction
• Plant growth hormones
• Khanh Hoa Province
• Somaclonal variations
• Phenotypic appearances
• Genetic stability
• Morphological and molecular markers
• Banana tissue culture propagated plantlets
• Clonal fidelity
• Assessment of clonal fidelity
• Genetic analysis
• Tissue culture raised banana
• Banana (Musa spp ) plant
• BMC Plant Biology
• Brassica napus
• Dwarfing genes
• Small up regulated response
• Community engagement
• Agricultural biotechnology
• Global health
• Global development
• Stakeholder engagement