tailieunhanh - In vivo and in vitro micrografting of Pistachio, Pistacia vera L. cv. siirt
In this study, the success of in vivo and in vitro micrografts of pistachio (Pistacia vera L. cv. "Siirt") materials are presented. The only variable tested was age (1, 5, 10, and 30-year-old trees). Ten- to 12-day-old axenic seedlings germinated in vitro or seedlings (3 to 5 months-old) grown in pots in vivo were used as rootstocks. Shoot tips collected from the four age classes of mature trees of pistachio were the source of scions. | Turk J Biol 27 (2003) 95-100 © TÜB‹TAK In Vivo and in Vitro Micrografting of Pistachio, Pistacia vera L. cv. “Siirt” Ahmet ONAY*, Vedat P‹R‹NÇ, Filiz ADIYAMAN, Çi¤dem IfiIKALAN, Engin T‹LKAT, Davut BAfiARAN Department of Biology, Faculty of Science and Literature, University Dicle, Diyarbak›r - TURKEY Received: Abstract: In this study, the success of in vivo and in vitro micrografts of pistachio (Pistacia vera L. cv. "Siirt") materials are presented. The only variable tested was age (1, 5, 10, and 30-year-old trees). Ten- to 12-day-old axenic seedlings germinated in vitro or seedlings (3 to 5 months-old) grown in pots in vivo were used as rootstocks. Shoot tips collected from the four age classes of mature trees of pistachio were the source of scions. Firm contact between the scion and rootstock was assured through the use of parafilm tape at the graft junction for in vivo micrografts. The in vivo micrografting system provided good growth and development for new axillary shoots. These plantlets were successfully transplanted and no problems were encountered with the establishment of micrografted plants in soil. The recovery of microscions was slow, but the use of micrografts onto herbaceous rootstocks proved a useful technique. Key Words: Micrografting, Pistacia vera L., Vegetative propagation Antepf›st›¤›n›n (Pistacia vera L. cv. "Siirt") ‹n Vitro ve ‹n Vivo Mikro Afl›lanmas› Özet: Bu çal›flmada Antep f›st›¤›n›n (Pistacia vera L. cv. "Siirt") in vitro ve in vivo mikro afl›lanmas› araflt›r›ld›. Çal›flmada farkl› yafl grubu a¤açlardan (1, 5, 10, 30 y›ll›k) al›nan mikroçeliklerin in vivo ve in vitro ortamda afl› tutma oranlar› rapor edildi. ‹n vitro mikro afl›lamada, laboratuvarda sterilize edilen tohumlardan, Murashige ve Skoog (MS) besi ortamlar›nda çimlendirilen 10-12 günlük fideler anaç olarak kullan›l›rken, in vivo mikro afl›lamada 3 ayl›k fideler anaç olarak kullan›ld›. ‹n vivo mikro afl›lamada, çelik ile anaç aras›ndaki destek ve kaynaflmay› .
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