tailieunhanh - Investigation of the antimicrobial activity of some Streptomyces isolates

Seventy-four different Streptomyces isolates were obtained from the soils of Mu¤la province. Antimicrobial activity was determined in of the isolates. Fifteen isolates showed strong activity against coagulase-negative Staphylococcus (CoNS). | Turk J Biol 27 (2003) 79-84 © TÜB‹TAK Investigation of the Antimicrobial Activity of Some Streptomyces Isolates Nurettin fiAH‹N* Biology Section, Faculty of Education, Mu¤la University, 48170, Kötekli, Mu¤la - TURKEY Aysel U⁄UR Department of Biology, Faculty of Arts and Sciences, Mu¤la University, 48170, Kötekli, Mu¤la - TURKEY Received: Abstract: Seventy-four different Streptomyces isolates were obtained from the soils of Mu¤la province. Antimicrobial activity was determined in of the isolates. Fifteen isolates showed strong activity against coagulase-negative Staphylococcus (CoNS). These isolates were extensively studied for their in vitro antimicrobial activity against Gram-positive and Gram-negative bacteria and yeasts. The results indicated that five isolates were highly active against CoNS and yeast with an inhibition zone at ≥ 20 mm. Three of these isolates were identified as Streptomyces antibioticus (MU106, MU107) and S. rimosus (MU114). The UV spectra of the culture extracts for the active isolates showed absorbance peaks ranging between 212 and 260 nm. Two bioactive regions were detected on the TLC plate (Rf and ). The UV spectrum of the active compounds in methanol showed peaks at 211 and 215 nm. Key Words: Antimicrobial activity, Antibiotics, Streptomyces, Actinomycetes Baz› Streptomyces ‹zolatlar›n›n Antimikrobiyal Aktivitelerinin Araflt›r›lmas› Özet: Mu¤la yöresi topraklar›ndan 74 farkl› Streptomyces izolat› elde edildi. ‹zolatlar›n % 'unda antimikrobiyal aktivite belirlendi. Onbefl izolat koagulaz-negatif Stafilokoklara (CoNS) karfl› güçlü antimikrobiyal aktivite gösterdi. Bu izolatlar›n Grampozitif, Gram-negatif bakteriler ve mayalara karfl› in vitro antimikrobiyal aktiviteleri kapsaml› olarak çal›fl›ld›. Sonuçlar göstermifltir ki, 5 izolat ≥ 20 mm inhibisyon zonuyla CoNS ve mayalara karfl› yüksek derecede aktiftir. Bu izolatlar›n üçü, Streptomyces antibioticus (MU106, MU107) ve S. rimosus (MU114) olarak .

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• Coagulase negative Staphylococcus
• Streptomyces antibioticus
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