tailieunhanh - Cytogenetic effects of copper chloride on the root tip cells of Helianthus annuus L.
The cytogenetic effects of copper chloride on root tip cells of Helianthus annuus were investigated. Seeds were treated with 10, 25, 50 and 100 mg/L (ppm) of copper chloride for 24 h. It was found that copper chloride has a marked mitodepressive action on mitosis. Mitotic abnormalities were increased and the mitotic index was decreased depending on the concentration of the copper chloride applied. | Turk J Biol 27 (2003) 43-46 © TÜB‹TAK Cytogenetic Effects of Copper Chloride on the Root Tip Cells of Helianthus annuus L. Hüseyin ‹NCEER*, Sema AYAZ, Osman BEYAZO⁄LU, Emine fiENTÜRK Department of Biology, Faculty of Arts and Sciences, Karadeniz Technical University, Trabzon - TURKEY Received: Abstract: The cytogenetic effects of copper chloride on root tip cells of Helianthus annuus were investigated. Seeds were treated with 10, 25, 50 and 100 mg/L (ppm) of copper chloride for 24 h. It was found that copper chloride has a marked mitodepressive action on mitosis. Mitotic abnormalities were increased and the mitotic index was decreased depending on the concentration of the copper chloride applied. Key Words: Copper chloride, Helianthus annuus, mitotic cell division, chromosomal anomalies Bak›r Klorür'ün Helianthus annuus L. Kök Ucu Hücreleri Üzerine Sitogenetik Etkileri Özet: Bak›r klorür'ün Helianthus annuus kök ucu hücreleri üzerine sitogenetik etkisi incelenmifltir. Tohumlar 10, 25, 50 ve 100 mg/L'l›k bak›r klorür çözeltisi ile 24 saat muamele edilmifltir. Bak›r klorür'ün mitoz bölünmeyi bask›lad›¤›, doz art›fl›na ba¤l› olarak kromozomal anormalliklere sebep oldu¤u ve mitotik indeksi (hücre bölünme frekans›) azaltt›¤› gözlenmifltir. Anahtar Sözcükler: Bak›r klorür, Helianthus annuus, mitoz bölünme, kromozomal anormallikler Introduction In developed agricultural systems, inorganic fertilisers are applied to the soil to supply the essential nutrients required for the growth of plants. However, the accumulation in the environment of heavy metals as a result of current agricultural systems is steadily increasing. Many cytological studies have been carried out to detect the harmful effects of various heavy metals on different plants (1-3). In addition, in developed industrial systems, industrial wastes affect genetic systems by producing various types of chromosomal abnormalities. Copper is potentially toxic to most organisms (4), which, at low .
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