tailieunhanh - Some properties of crude carboxymethyl cellulase of aspergillus niger Z10 wild type strain

A carboxymethyl cellulase enzyme was prepared from a wild type strain of Aspergillus niger Z10. Analyses of the enzyme preparation by SDS-PAGE revealed two protein bands showing cellulolytic activity. The molecular weight of these bands was estimated to be around 83,000 and 50,000. | Turk J Biol 26 (2002) 209-213 © TÜB‹TAK Some Properties of Crude Carboxymethyl Cellulase of Aspergillus niger Z10 Wild-Type Strain Gökhan CORAL* Mersin University, Faculty of Arts and Sciences, Department of Biology, 33342, Çiftlikköy, Mersin - TURKEY Burhan ARIKAN Çukurova University, Faculty of Arts and Sciences, Department of Biology, 01330, Balcal›, Adana - TURKEY M. Nisa ÜNALDI Mustafa Kemal University, Faculty of Arts and Sciences, Department of Biology, 31040, Hatay - TURKEY Hatice GÜVENMEZ Çukurova University, Faculty of Arts and Sciences, Department of Biology, 01330 Balcal›, Adana - TURKEY Received: Abstract: A carboxymethyl cellulase enzyme was prepared from a wild type strain of Aspergillus niger Z10. Analyses of the enzyme preparation by SDS-PAGE revealed two protein bands showing cellulolytic activity. The molecular weight of these bands was estimated to be around 83,000 and 50,000. The optimum temperature of the enzyme was observed to be around 40 °C. It was found that the enzyme’s activity has a broad pH range between 3 to 9 and of the original activity was retained after heat treatment at 90 °C for 15 min. Key Words: Aspergillus niger, Carboxymethyl cellulase (CMCase) Aspergillus niger Z10 Yaban Tip Suflundan Elde Edilen Ham Karboksimetil Selülaz’›n Baz› Özellikleri Özet: Aspergillus niger Z10 yaban tip suflundan karboksimetil selülaz enzimi haz›rlanm›flt›r. Enzim preparasyonunun SDS-PAGE analizi sonucunda, jel üzerinde selülolitik aktivite gösteren iki protein band› saptanm›flt›r. Bu bandlar›n moleküler a¤›rl›klar› yaklafl›k olarak s›ras›yla 83000 ve 50000 olarak hesaplanm›flt›r. Enzimin optimum s›cakl›¤›n›n 40 °C civar›nda oldu¤u gözlenmifltir. Enzimin aktivite gösterdi¤i pH aral›¤›n›n ise 3 ile 9 aras›nda oldu¤u bulunmufl ve enzim 90 °C'de 15 dakika tutuldu¤unda, orijinal aktivitesinin % 'nin devam etti¤i kaydedilmifltir. Anahtar Sözcükler: Aspergillus niger, Karboksimetil selülaz (CMCaz) Introduction Cellulose is .