tailieunhanh - Mutagenesis of the tal gene encoding transaldolase in the Cyanobacterium, Anabaena sp. PCC7120

The transaldolase gene (tal) of Anabaena sp. PCC7120 was interrupted by the insertion of the interposon Ω. Transaldolase assays showed that the tal mutant strain possessed the same activity as the wild-type, indicating that the second copy of the gene complements the enzyme activity. | Turk J Biol 32 (2008) 135-141 © TÜB‹TAK Mutagenesis of the tal gene-encoding Transaldolase in the Cyanobacterium, Anabaena sp. PCC7120 Haydar KARAKAYA, Nicholas H. MANN Department of Biological Sciences, University of Warwick, Coventry CV4 7AL - UK Received: Abstract: The transaldolase gene (tal) of Anabaena sp. PCC7120 was interrupted by the insertion of the interposon Ω. Transaldolase assays showed that the tal mutant strain possessed the same activity as the wild-type, indicating that the second copy of the gene complements the enzyme activity. Being coded by a gene (zwf) downstream of tal and probably in the same operon, glucose-6-phosphate dehydrogenase (G6PDH) activity was also analysed. Only 34% of wild-type G6PDH activity was retained in the tal mutant strain. This may have due to the polar affect of tal mutation on the transcription of the zwf gene. Growth of the tal mutant was not different than that of the wild-type in the presence of combined nitrogen, but the mutant reached the stationary phase faster than the wild-type in the absence of combined nitrogen. This was probably because of the reduction of G6PDH activity, resulting in less production of reductant and energy in heterocysts, which negatively affects nitrogen fixation and growth. Key Words: Transaldolase mutant, tal mutation, glucose-6-phosphate dehydrogenase, Anabaena Siyanobakteri Anabaena sp. PCC7120 Suflunun Transaldolaz› Kodlayan tal Geninin Mutasyonu Özet: Anabaena sp. PCC7120 transaldolaz geni (tal) Ω interpozonu eklenerek mutasyona u¤rat›lm›flt›r. Transaldolaz testleri mutant ve yabani tip hücrelerin ayn› düzeyde aktiviteye sahip oldu¤unu göstermifltir. Bu sonuç bu genin genomdaki di¤er kopyas›n›n enzim aktivitesini tamamlad›¤›n› göstermektedir. tal geninin afla¤›s›nda ve muhtemelen ayn› operonda yer alan bir gen (zwf) taraf›ndan kodlanmas› nedeniyle ayr›ca glukoz-6-fosfat dehidrogenaz (G6PDH) aktivitesi de test edilmifltir. Bu tal mutant suflta G6PDH aktivitesinin sadece .

• Sinh học
• Transaldolase mutant
• Tal mutation
• Glucose 6 phosphate dehydrogenase
• Interposon Ω
• Enzyme activity
• Crpping system
• Soil enzyme activity
• Different cropping system
• Important soil enzyme activity
• Organic carbon
• Microbial activity with different depth
• Antioxidant enzymes
• Temperature induction response
• Temperature enhances antioxidant enzyme activity
• Osmoprotectants in tomato
• Early postemergence
• Greengram (Vigna radiata L )
• Nutrient uptake by weeds
• Compacting of soil
• Urease enzyme activity
• Carbon dioxide evaluation
• Nitrogen mineralisation
• Angiotensin converting enzyme inhibitory activity
• Vietnamese medicinal plants
• Angiotensin 1 converting enzyme
• Leea rubra
• Uncaria sessilifructus
• Residue management
• Soil enzyme
• Soil quality
• Diverse cropping systems
• Residue management practices
• Trichoderma reesei
• Chitinase enzyme
• CDR Design
• Potato dextrose agar
• Enhance chitinase enzyme activity
• Gamma radiation induced mutagenesis
• Candida albicans
• Secreted aspartyl proteinase activity
• Acid proteinase
• Bloodstream identified
• Microbial biomass carbon
• Enzymatic activity
• Typic haplustepts
• INM on soil microbial biomass carbon
• Enzyme activity under soybean
• L glutaminase
• pH on soil L glutaminase activity
• Soil L glutaminase activity
• Soil temperature
• Biochemistry
• molecular Biology
• biological cells
• chymotrypsin C
• Scientific reports
• Optimal pH
• Partial purification
• Intestinal triglyceride lipase
• Polyethylene glycol
• Conventional management
• Microbial biomass
• Organic management
• Vineyard soils
• Acute toxicity
• Chlorpyrifos on growth
• Chlorophyll a synthesis
• Cyanobacterium spirulina (Arthrospira) platensis
• Micronutrients on growth attributes
• Yield and soil enzyme activity
• Elephant foot yam
• Viable solution
• Important food crop
• L asparaginase
• Electrical conductivity
• Soil enzyme l asparaginase activity
• Physico chemical properties
• Dry DSR
• Soil microbial population
• Grain yield
• Dry direct seeded rice
• Organic manures
• Available nutrients
• Rice rice cropping system
• Long term use of inorganic fertilizers
• Combination on soil properties
• α galactosidase
• β glucosidase
• Fermented food
• Traditional fermented foods
• β glucosidase enzyme activity
• Lactic strains isolated
• BMC Cancer
• Cervical cancer
• Non enzymatic functions
• Cancer growth
• Quality protein maize
• Potassium and sulphur
• Nutrient application
• Oil palm kernel meal
• De oiled oil palm kernel meal based
• Biofloc system on growth
• Digestive enzyme activity
• Pacific white shrimp
• Litopenaeus Vannamei
• Pangasianodon hypophthlmus
• Effects of thermal stress
• Dietary zinc on growth performance
• Superoxide dismutase–1
• Catalase enzyme activity
• Pangasianodon hypophthalmus
• Ligninolytic enzymes
• Lignin peroxidase
• manganese peroxidase
• Five bacterial strains
• Culture conditions on ligninolytic enzyme
• Activity of five bacterial strains
• Journal of Chemistry
• PEGylated quinoline
• Colon cancer
• P glycoprotein
• Cytochrome P450 enzyme
• Polyethylene glycol (PEG)
• Polymeric carriers conjugated