tailieunhanh - Increasing the injured area on hypocotyl explants of flax (Linum usitatissimum L.) leads to high frequency callus based shoot regeneration

In this study, it was aimed to increase the frequency of shoots, which would be nominated to be transgenic after Agrobacterium inoculation, through expanding the wounding area by peeling the epidermis layer of hypocotyl explants of flax plants. | Turk J Biol 26 (2002) 95-98 © TÜB‹TAK Increasing the Injured Area on Hypocotyl Explants of Flax (Linum usitatissimum L.) Leads to High Frequency Callus-Based Shoot Regeneration Mustafa YILDIZ, Celâl ER . Faculty of Agriculture, Department of Field Crops, 06110 Ankara-TURKEY Received: Abstract: In this study, it was aimed to increase the frequency of shoots, which would be nominated to be transgenic after Agrobacterium inoculation, through expanding the wounding area by peeling the epidermis layer of hypocotyl explants of flax plants. Callus weight, percentage of hypocotyls producing shoots, shoot number per explant and total shoot number per petri dish were recorded as g, 40%, and , respectively in non-peeled explants. On the other hand, when the epidermis layer was peeled, callus weight, percentage of hypocotyls producing shoots, shoot number per explant and total shoot number per petri dish increased to g, , and , respectively. Key Words: Flax, injured area, callus-based shoot regeneration Keten (Linum usitatissimum L.) Hipokotil Eksplantlar›nda Yaralanan Yüzeyin Art›r›lmas› Kallustan Geliflen Sürgün Frekans›n› Yükseltmektedir Özet: Bu araflt›rmada, keten bitkisinin hipokotil eksplantlar›nda, epidermis tabakas› soyularak yaralanan yüzeyin geniflletilmesi ile Agrobacterium inokulasyonundan sonra transgenik olmaya aday sürgün frekans›n›n art›r›lmas› amaçlanm›flt›r. Soyulmam›fl hipokotil eksplantlar›nda kallus a¤›rl›¤›, sürgün oluflturan hipokotil yüzdesi, eksplant bafl›na sürgün say›s› ve petrideki sürgün say›s› s›ras›yla g, %40, adet ve adet olarak belirlenmifltir. Di¤er taraftan, epidermis tabakas› soyuldu¤unda, kallus a¤›rl›¤›, sürgün oluflturan hipokotil yüzdesi, eksplant bafl›na sürgün say›s› ve petrideki sürgün say›s› s›ras›yla g, %, adet ve adete yükselmifltir. Anahtar Sözcükler: Keten, yaralanan yüzey, kallus tabanl› sürgün rejenerasyonu Introduction Several genes that