tailieunhanh - Effect of some biocides on glutathione-s-transferase in barley, wheat, lentil and chickpea plants

The conjugation of glutathione with herbicides and insecticides is considered to be one of the major detoxification mechanisms of plants. This conjugation is catalysed by a family of multifunctional proteins known as glutathione-s-transferases. Treatment of wheat (Triticum aestivum L. cv. Cumhuriyet-75), barley (Hordeum vulgare L. cv. Kaya), chickpea (Cicer arietinum L. cv. Ispanyol). | Turk J Biol 26 (2002) 89-94 © TÜB‹TAK Effect of Some Biocides on Glutathione-s-transferase in Barley, Wheat, Lentil and Chickpea Plants Gülseren COfiKUN, Figen Z‹HN‹O⁄LU Ege University, Faculty of Science, Department of Biochemistry, 35100 Bornova-‹zmir - TURKEY Received: Abstract: The conjugation of glutathione with herbicides and insecticides is considered to be one of the major detoxification mechanisms of plants. This conjugation is catalysed by a family of multifunctional proteins known as glutathione-s-transferases. Treatment of wheat (Triticum aestivum L. cv. Cumhuriyet-75), barley (Hordeum vulgare L. cv. Kaya), chickpea (Cicer arietinum L. cv. Ispanyol), and lentil (Lens culinaris Medik. cv. K›fll›k-k›rm›z›-51) with the selective herbicides 2,4-D (2,4-Dichloro phenoxyacetic acid) and linuron (N-(3,4-dichlorophenyl)-N´-methoxy-N´-methyl urea), and insecticides parathion methyl (O,O-dimethyl O-(4nitrophenyl) phosphorothioate) and malathion (S-1,2-bis(ethoxycarbonyl)ethyl O,O-dimethyl phosphorodithioate) caused a progressive increase in glutathione-s-transferase activity in roots and shoots. The maximum increase in enzyme activity was observed in parathion methyl treated wheat roots: 485% of control with a parathion methyl concentration of mM. A similar but lower increase in glutathione (GSH) levels was observed for roots and shoots, differing according to the origin and the type of pesticide. Key Words: Glutathione-s-transferase, detoxification, pesticide Arpa, Bu¤day, Mercimek ve Nohut Bitkilerindeki Glutation-s-transferaz Üzerine Baz› Biosidlerin Etkisi Özet: Glutationun herbisitler ve insektisitlerle konjugasyonu, bitkilerin detoksifikasyon mekanizmalar›n›n en önemlilerinden biridir. Bu konjugasyon, glutation-s-transferazlar olarak bilinen multifonksiyonel bir enzim ailesi taraf›ndan katalizlenir. Bu¤day (Triticum aestivum ), arpa (Hordeum vulgare ), nohut (Cicer arietinum .‹spanyol) ve mercime¤in .

• Sinh học
• Glutathione s transferase
• Chickpea plants
• Detoxification mechanisms of plants
• Multifunctional proteins
• Wheat Triticum aestivum L