tailieunhanh - Purification and determination of the molecular structure of hemolymph lectin of Agrotis segetum (Denis and Schiff.)

Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) showed a single protein band with a molecular weight of 69,000 in both the presence and absence of 2-Mercaptoethanol. Alternatively, the molecular weight of this lectin was estimated to be 69,000 by gel filtration on Superdex200. | Turk J Biol 26 (2002) 49-55 © TÜB‹TAK Purification and Determination of the Molecular Structure of Hemolymph Lectin of Agrotis segetum (Denis and Schiff.) Nursel GÜL, Cevat AYVALI Department of Biology, Science Faculty of Ankara University, 06100, Beflevler, Ankara - TURKEY Received: Abstract: A soluble lectin was purified from the hemolymph of Agrotis segetum by two methods, via affinity chromatography on a Sepharose-4B column and gel filtration on a Superdex-200 column. Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) showed a single protein band with a molecular weight of 69,000 in both the presence and absence of 2Mercaptoethanol. Alternatively, the molecular weight of this lectin was estimated to be 69,000 by gel filtration on Superdex200. Electron microscopic observation of the purified lectin was reticular in structure and consisted of tandem aligned spherical basic units. Key Words: Agrotis segetum, lectin (agglutinin), purification, molecular weight, transmission electron microscopy (TEM) Agrotis segetum (Denis ve Schiff.) Hemolenf Lektininin Saflaflt›r›lmas› ve Moleküler Yap›s›n›n Belirlenmesi Özet: Agrotis segetum hemolenfinden çözünebilir lektin, Sepharose-4B kolonunda affinite kromatografisi ve Süperdex–200 kolonunda jel filtrasyonu metodlar›yla saflaflt›r›lm›flt›r. SDS-poliakrilamid jel elektroforezi 2-Merkaptoetanolllü ve 2Merkaptoetanolsüz olarak yaklafl›k 69,000 molekül a¤›rl›¤›nda bir protein band› göstermifltir. Superdex-200 jel filtrasyonuyla lektinin yaklafl›k molekül a¤›rl›¤›n›n 69,000 oldu¤u tespit edilmifltir. Saflaflt›r›lan lektinin a¤s› (retiküler) bir yap›da oldu¤u ve birbiri ard›na dizilmifl küresel birim yap›lardan meydana geldi¤i elektron mikroskobunda gözlenmifltir. Anahtar Sözcükler: Agrotis segetum, lektin (aglutinin), saflaflt›rma, molekül a¤›rl›¤›, geçirmeli elektron mikroskobu (TEM) Introduction Lectins (agglutinins) are cell-agglutinating proteins or glycoproteins of non-immune origin that .

• Sinh học
• Agrotis segetum
• Molecular weight
• Transmission electron microscopy
• Gel filtration on Superdex200
• Polyacrylamide gel electrophoresis
• Bacterial flora
• Insecticidal activity
• Agrotis segetum Schiff
• Safe biological control agent
• Vegetable fields
• Steinernima and heterorhabditis black cutworm
• Potato crop
• Five days of treatment
• Reduced number of portal entries
• Entomopathogenic nematode
• Steinernema websteri
• Pest control
• Fruits worldwide