tailieunhanh - Typing of some clinically significant nocardia strains using a digoxigenin labelled rDNA gene probe

In this study, ribotyping (restriction polymorphism ribosomal RNA analyses) was applied as a taxonomic tool for bacteria belonging to the genus Nocardia. A total of 21 Nocardia strains were clearly distinguished using a combination of EcoRV gene restriction endonuclease patterns together with a digoxigenin-labelled Streptomyces violaceoruber TK21 rDNA probe. | Turk J Biol 26 (2002) 1-8 © TÜB‹TAK Typing of Some Clinically Significant Nocardia Strains Using a Digoxigenin-Labelled rDNA Gene Probe Kamil IfiIK, Nevzat fiAH‹N, Ergin KAR‹PTAfi University of Ondokuz May›s, Faculty of Arts and Science, Department of Biology, 55139, Kurupelit, Samsun - TURKEY Michael GOODFELLOW* Newcastle University, The Medical School, Department of Microbiology, Newcastle, NE2 4HH, . Received: Abstract: In this study, ribotyping (restriction polymorphism ribosomal RNA analyses) was applied as a taxonomic tool for bacteria belonging to the genus Nocardia. A total of 21 Nocardia strains were clearly distinguished using a combination of EcoRV gene restriction endonuclease patterns together with a digoxigenin-labelled Streptomyces violaceoruber TK21 rDNA probe. Five distinct ribotypes were identified, and each ribotype contained 4 to 8 restriction fragments ranging in size from to kb. The evaluation of the results showed that N. brasiliensis, N. farcinica, N. otitidiscaviarum and N. seriolea are taxonomically wellestablished species, except N. pseudobrasiliensis. Therefore, it can be concluded that ribotyping technique provide a useful information for the classification of clinically significant Nocardia. Key Words: Nocardia, Ribotyping, Taxonomy Klinik Önemi Olan Baz› Nocardia Sufllar›n›n Digoksijeninle ‹flaretlenmifl Ribozomal DNA Probu Kullan›lmas›yla Tiplendirilmesi Özet: Bu çal›flmada ribotyping metodu, Nocardia cinsine ait bakterilerin ay›rtedilmesinde, taksonomik bir yöntem olarak uygulanm›flt›r. Toplam 21 Nocardia suflu, Streptomyces violaceoruber TK21 suflundan elde edilen digoksijeninle iflaretlenen ribosomal DNA probu ve EcoRV endonukleaz enziminin birlikte kulan›lmas›yla aç›k bir flekilde birbirlerinden ay›rtedilebilmifllerdir. Herbiri ile kb ölçüm de¤erinde ve 4 ile 8 fragmente sahip birbirinden ayr›lan befl ribotip belirlenmifltir. Sonuçlar›n de¤erlendirilmesi, N. pseudobrasiliensis d›fl›nda N. .

• Sinh học
• Ribosomal RNA analyses
• Genus Nocardia
• EcoRV gene
• Digoxigenin labelled Streptomyces
• TK21 rDNA probe
• Ribosomal RNA analysis
• Glucoseyeast extract agar plates
• Genomic DNA
• Streptomyces violaceoruber TK21 rDNA
• Pathogenic nocardiae