tailieunhanh - Susceptibility of different bean (Phaseolus vulgaris L.) cultivars to agrobacterium tumefaciens

Although several direct gene transfer techniques have been developed, Agrobacterium tumefaciens is still preferred for the transfer of foreign genes into many plant species. Perhaps the greatest weakness of A. tumefaciens is the host range limitation. | Turk J Biol 25 (2001) 447-452 © TÜB‹TAK Susceptibility of Different Bean (Phaseolus vulgaris L.) Cultivars to Agrobacterium tumefaciens Aziz KARAKAYA Ankara Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü, D›flkap›, 06110, Ankara - TURKEY Sebahattin ÖZCAN Ankara Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, D›flkap›, 06110, Ankara - TURKEY Received: Abstract : Although several direct gene transfer techniques have been developed, Agrobacterium tumefaciens is still preferred for the transfer of foreign genes into many plant species. Perhaps the greatest weakness of A. tumefaciens is the host range limitation. Therefore, in this study, the susceptibility of different bean cultivars to oncogenic A. tumefaciens strain A281 was assessed. The cultivars 4F-2409, Karacaflehir-90, Akman-98 and Eskiflehir-855 resulted in higher frequencies of tumor formation than the other cultivars tested. Tumor formation was not observed in Afyon-3, IVD-10, Göynük-98, Yunus-90 or fiehirali-90 bean cultivars. Tumor formation was observed on both the hypocotyl and epicotyl portions of the plants. Key Words: Agrobacterium tumefaciens, gene transfer, tumor formation, bean Farkl› Fasulye (Phaseolus vulgaris L.) Çeflitlerinin Agrobacterium tumefaciens’e karfl› Duyarl›l›klar›n›n Belirlenmesi Özet : Farkl› do¤rudan gen aktar›m teknikleri gelifltirilmesine karfl›n, Agrobacterium tumefaciens birçok bitki türüne yabanc› genlerin aktar›m›nda tercih edilmektedir. Belki de A. tumefaciens’in en büyük dezavantaj› konukçusunun s›n›rl› olmas›d›r. Bu yüzden, bu çal›flmada farkl› fasulye çeflitlerinin do¤al tip A. tumefaciens streyni A281’e karfl› duyarl›l›klar› belirlenmifltir. 4F-2409, Karacaflehir-90, Akman-98 ve Eskiflehir 855 çeflitleri test edilen di¤er çeflitlere oranla daha yüksek oranlarda ur oluflturmufllard›r. Afyon-3, IVD-10, Göynük-98, Yunus-90,ve fiehirali-90 çeflitlerinde ur oluflumu gözlenmemifltir. Bitkilerin hem hipokotil ve hem de epikotil k›s›mlar›nda ur .