tailieunhanh - Callus induction and plant regeneration from mature embryos of oat (Avena sativa L.)

The aim of this study, which was conducted in Ankara University, Faculty of Agriculture, Department of Field Crops, biotechnology laboratory in 1998-1999, was to determine the callus induction and plant regeneration capacity of oat (Avena sativa L.). | Turk J Biol 25 (2001) 427-434 © TÜB‹TAK Callus Induction and Plant Regeneration from Mature Embryos of Oat (Avena sativa L.) Melahat A. B‹RS‹N Ankara University, Faculty of Agriculture, Department of Field Crops, 06110 D›flkap›, Ankara - TURKEY Sertaç ÖNDE Middle East Technical University, Faculty of Arts and Sciences, Department of Biological Sciences, 06531, Ankara - TURKEY Murat ÖZGEN Ankara University, Faculty of Agriculture, Department of Field Crops, 06110 D›flkap›, Ankara - TURKEY Received: Abstract: The aim of this study, which was conducted in Ankara University, Faculty of Agriculture, Department of Field Crops, biotechnology laboratory in 1998-1999, was to determine the callus induction and plant regeneration capacity of oat (Avena sativa L.). The mature embryos of 10 oat genotypes were utilised for callus induction. The statistical analysis of the results revealed that callus induction and plant regeneration capacity were dependent on the oat genotype. However, a significant relationship was not established between callus induction and regeneration capacity and, furthermore, the number of regenerated plants was determined to be directly related to the callus production capacity. Key Words: Avena sativa L., plant regeneration, embryo culture, callus induction Yulafta (Avena sativa L.) Olgun Embriyolardan Kallus Oluflumu ve Bitki Rejenerasyonu Özet: Ankara Üniversitesi Ziraat Fakültesi Tarla Bitkileri Bölümü biyoteknoloji laboratuvar›nda 19981999 y›llar›nda yap›lan bu araflt›rman›n amac›; yulafta kallus oluflumu ve rejenerasyon yetene¤ini belirlemektir. On yulaf çeflit ya da hatt›n›n kullan›ld›¤› araflt›rmada; kallus oluflturmada olgun embriyolardan yararlan›lm›flt›r. Elde edilen veriler istatistik olarak de¤erlendirildi¤inde; kallus oluflumu ve rejenerasyon kapasitesinin çeflit ve hatlara göre de¤iflti¤i, kallus oluflumu ve rejenerasyon kapasitesi aras›nda önemli bir iliflkinin olmad›¤›; rejenere bitki say›s›n›n do¤rudan kallus .

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