tailieunhanh - Pulsed field gel electrophoresis (PFGE) technique and its use in molecular biology
In recent years, the use of pulsed-field gel electrophoresis (PFGE) in the molecular biology area has been subject to much research. PFGE is a powerful tool for characterizing various strains at the DNA level, obtaining relevant information on genome size and constructing the physical and genetic map of the chromosome of bacteria that are poorly understood at the genetic level as well as in separating chromosomes in microorganisms, and in the long-range mapping of mammalian genes. | Turk J Biol 25 (2001) 405-418 © TÜB‹TAK Pulsed-Field Gel Electrophoresis (PFGE) Technique and its use in Molecular Biology Esin (HACIO⁄LU) BASIM Süleyman Demirel University, Faculty of Agriculture, Department of Plant Protection, 32260 Çünür, Isparta - TURKEY Hüseyin BASIM Akdeniz University, Faculty of Agriculture, Department of Plant Protection, 07058, Antalya - TURKEY Received: Abstract: In recent years, the use of pulsed-field gel electrophoresis (PFGE) in the molecular biology area has been subject to much research. PFGE is a powerful tool for characterizing various strains at the DNA level, obtaining relevant information on genome size and constructing the physical and genetic map of the chromosome of bacteria that are poorly understood at the genetic level as well as in separating chromosomes in microorganisms, and in the long-range mapping of mammalian genes. PFGE also has advantage of examining the elongated and oriented configuration of large DNA molecules in agarose gels at finite field strengths. In this review, the use of PFGE in molecular biology, the general characteristics of PFGE, different types of PFGE and factors affecting PFGE are introduced. Key Words: Pulsed-Field Gel Electrophoresis (PFGE), CHEF, Molecular Biology, Biotechnology, Restriction Enzymes. Pulsed-Field Jel Elektroforez (PFGE) Tekni¤i ve Moleküler Biyoloji Alan›nda Kullan›m› Özet: Son y›llarda, moleküler biyoloji alan›nda pulsed-field jel elektroforez (PFGE)’in kullan›m› birçok araflt›rmaya konu olmufltur. PFGE, DNA düzeyinde çeflitli strainlerin karakterize edilmesinde, genom büyüklükleri hakk›nda bilgi elde etmede, genetik düzeyde anlafl›lamam›fl bakteri kromozomlar›n›n fiziki ve genetik haritalar›n›n oluflturulmas›nda,mikroorganizmalar›n kromozomlar›n›n ayr›lmas›nda, ve memeli genlerinin büyük çaptaki haritalanmas›nda kullan›lan etkili bir metottur. PFGE; agaroz jel içerisindeki büyük DNA moleküllerinin uygun bir alanda de¤iflik flekillerde hareket etmesini .
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