tailieunhanh - In vitro antimicrobial activity and phytochemical analysis of some indian medicinal plants

The antibacterial effect of some selected Indian medicinal plants was evaluated on bacterial strains like Bacillus cereus ATCC11778, Staphylococcus aureus ATCC25923, Enterobacter aerogenes ATCC13048, Escherichia coli ATCC25922 and Klebsiella pneumoniae NCIM2719. The solvents used for the extraction of plants were water and methanol. | Turk J Biol 31 (2007) 53-58 © TÜB‹TAK In vitro Antimicrobial Activity and Phytochemical Analysis of Some Indian Medicinal Plants Jigna PAREKH, Sumitra V. CHANDA Phytochemical, Pharmacological and Microbiological Laboratory, Department of Biosciences, Saurashtra University, Rajkot 360 005 - INDIA Received: Abstract: The antibacterial effect of some selected Indian medicinal plants was evaluated on bacterial strains like Bacillus cereus ATCC11778, Staphylococcus aureus ATCC25923, Enterobacter aerogenes ATCC13048, Escherichia coli ATCC25922 and Klebsiella pneumoniae NCIM2719. The solvents used for the extraction of plants were water and methanol. The in vitro antibacterial activity was performed by agar disc diffusion and agar well diffusion method. The most susceptible Gram-positive bacteria was B. cereus, while the most susceptible Gram-negative bacteria was K. pneumoniae. The extracts of Abrus precatorius, Cardiospermum halicacabum and Gmelina asiatica could not inhibit any of the bacterial strains investigated. The most active antibacterial plant was Caesalpinia pulcherrima. The significant antibacterial activity of active extracts was compared with the standard antimicrobics, piperacillin (100 µg/disc) and gentamicin (10 µg/disc). The results obtained in the present study suggest that Caesalpinia pulcherrima can be used in treating diseases caused by the test organisms. Key Words: Medicinal plants, antibacterial activity, aqueous extract, methanol extract Introduction Infectious diseases are the leading cause of death world-wide. Antibiotic resistance has become a global concern (1). The clinical efficacy of many existing antibiotics is being threatened by the emergence of multidrug-resistant pathogens (2). Many infectious diseases have been known to be treated with herbal remedies throughout the history of mankind. Natural products, either as pure compounds or as standardized plant extracts, provide unlimited opportunities for new drug leads .