tailieunhanh - Variation of callus induction through anther culture in water chestnut (Trapa sp.)

Effects of culture media and genotypes of water chestnut (Trapa sp.) on the frequency of callus induction from anther culture were investigated. Results revealed that the N6 media supplemented with mg/l BA, mg/l NAA and mg/l GA3 were suitable for callus induction. | Turk J Biol 31 (2007) 41-45 © TÜB‹TAK Variation of Callus Induction Through Anther Culture in Water Chestnut (Trapa sp.) Aminul HOQUE1, Manosh Kumar BISWAS2, Shamiul ALAM1 1 Department of Agronomy and Agriculture Extension, University of Rajshahi, Rajshahi, 6205 - BANGLADESH 2 Department of Botany, University of Rajshahi, Rajshahi, 6205 - BANGLADESH Received: Abstract: Effects of culture media and genotypes of water chestnut (Trapa sp.) on the frequency of callus induction from anther culture were investigated. Results revealed that the N6 media supplemented with mg/l BA, mg/l NAA and mg/l GA3 were suitable for callus induction. Callus yielded poorly in MS-containing media compared to that of N6 media. No callus was induced on 2, 4-D containing media. Among the tested 18 genotypes, only 15 genotypes produced calli. Key Words: Anther culture, callus, water chestnut Introduction Water chestnut is an aquatic plant belonging to the Trapaceae family. It is popular for its edible starchy fruits. The fruit contains about 80% starch, 5% protein and a significant amount of vitamins (1,2). It has been commercially cultivated in India, China and Italy for its edible fruits in water bodies on low and plane lands or lakes (3,4,5). Some species are often cultivated in paddy fields in place of rice (6,7,8). Anther culture is used to produce haploid plants. The technique was first described by Guha and Maheshwari in 1964. They reported direct development of embryos from microspores of Datura innoxia by the culture of excised anthers (9). Anther culture has been used for crop improvement and the development of cultivars. This technique has some advantages over the conventional breeding such as its fast regeneration efficiency of desired genotypes (10,11,12). This technique also opened a new avenue of breeding system that covers resistance and quality plant development, shortens the existing breeding cycle with immediate fixation of homozygosity, .