tailieunhanh - Assessment of polymorphic AFLP markers in Triticum durum and Aegilops sp.

Compared to other Polymerase Chain Reaction (PCR) based molecular markers, the Amplified Fragment Length Polymorphism (AFLP) marker system probably requires more technical expertise for the sequential enzymatic reaction steps. | Turk J Biol 25 (2001) 291-299 © TÜB‹TAK Assessment of Polymorphic AFLP Markers in Triticum durum and Aegilops sp. Aytül ‹NC‹RL‹, Hatice B‹LG‹Ç, Mahinur S. AKKAYA Middle East Technical University, Department of Chemistry, Ankara, TR-06531, TURKEY Received: Abstract: Compared to other Polymerase Chain Reaction (PCR) based molecular markers, the Amplified Fragment Length Polymorphism (AFLP) marker system probably requires more technical expertise for the sequential enzymatic reaction steps. In addition, the reproducibility of the AFLP marker patterns is much more dependent on the quality and the purity of the DNA samples to be processed. The present study describes the optimization of reaction conditions and determination of the most polymorphic selective amplification primer sets in a comparison of winter and spring type Turkish durum wheat varieties. AFLP products resulted in about 100 bands, and the number of polymorphic AFLP loci obtained ranged from 6 to 32 per selective primer combination. Among the nine Aegilops accessions, at a single combination, 137 polymorphic loci were observed. Key Words: Aegilops, AFLP, DNA fingerprinting, durum wheat Bu¤dayda ve Aegilops'ta Polimorfik AFLP Belirleyicilerinin De¤erlendirilmesi Di¤er Polimeraz Zincir reaksyonu (PZR) tabanl› moleküler belirleyicilere k›yasla, Ço¤alt›lm›fl Parça Uzunluk Polimorfizm (ÇPUP) belirleyici sistemi birbirini izleyen enzim reaksiyonlar›ndan ötürü daha çok teknik deneyim gerektirmektedir. Bununla birlikte ÇPUP belirleyicilerinin tekrarlanabilir sonuçlar üretebilmesi DNAn›n safl›¤›na ve kalitesine ba¤l›d›r. Bu çal›flma reaksiyon koflullar›n›n optimizasyonunu ve en polimorfik seçici ço¤altma aflamas›ndaki primer setlerinin bir k›fll›k ve bir yazl›k Türk durum bu¤day› çeflitinde saptanmas›n› içermektedir. ÇPUP ürünleri 100 civar›nda bant vermifltir. Seçici ço¤altma primer setlerinde polimorfik ÇPUP lokuslar› 6 ile 32 aras›nda de¤iflmifltir. Dokuz aegilops akseyonunda ise bir primer .