tailieunhanh - Assessment of polymorphic AFLP markers in Triticum durum and Aegilops sp.

Compared to other Polymerase Chain Reaction (PCR) based molecular markers, the Amplified Fragment Length Polymorphism (AFLP) marker system probably requires more technical expertise for the sequential enzymatic reaction steps. | Turk J Biol 25 (2001) 291-299 © TÜB‹TAK Assessment of Polymorphic AFLP Markers in Triticum durum and Aegilops sp. Aytül ‹NC‹RL‹, Hatice B‹LG‹Ç, Mahinur S. AKKAYA Middle East Technical University, Department of Chemistry, Ankara, TR-06531, TURKEY Received: Abstract: Compared to other Polymerase Chain Reaction (PCR) based molecular markers, the Amplified Fragment Length Polymorphism (AFLP) marker system probably requires more technical expertise for the sequential enzymatic reaction steps. In addition, the reproducibility of the AFLP marker patterns is much more dependent on the quality and the purity of the DNA samples to be processed. The present study describes the optimization of reaction conditions and determination of the most polymorphic selective amplification primer sets in a comparison of winter and spring type Turkish durum wheat varieties. AFLP products resulted in about 100 bands, and the number of polymorphic AFLP loci obtained ranged from 6 to 32 per selective primer combination. Among the nine Aegilops accessions, at a single combination, 137 polymorphic loci were observed. Key Words: Aegilops, AFLP, DNA fingerprinting, durum wheat Bu¤dayda ve Aegilops'ta Polimorfik AFLP Belirleyicilerinin De¤erlendirilmesi Di¤er Polimeraz Zincir reaksyonu (PZR) tabanl› moleküler belirleyicilere k›yasla, Ço¤alt›lm›fl Parça Uzunluk Polimorfizm (ÇPUP) belirleyici sistemi birbirini izleyen enzim reaksiyonlar›ndan ötürü daha çok teknik deneyim gerektirmektedir. Bununla birlikte ÇPUP belirleyicilerinin tekrarlanabilir sonuçlar üretebilmesi DNAn›n safl›¤›na ve kalitesine ba¤l›d›r. Bu çal›flma reaksiyon koflullar›n›n optimizasyonunu ve en polimorfik seçici ço¤altma aflamas›ndaki primer setlerinin bir k›fll›k ve bir yazl›k Türk durum bu¤day› çeflitinde saptanmas›n› içermektedir. ÇPUP ürünleri 100 civar›nda bant vermifltir. Seçici ço¤altma primer setlerinde polimorfik ÇPUP lokuslar› 6 ile 32 aras›nda de¤iflmifltir. Dokuz aegilops akseyonunda ise bir primer .

• Sinh học
• DNA fingerprinting
• Durum wheat
• Triticum durum
• Aegilops sp
• Polymorphic AFLP markers
• Polymerase chain reaction
• SSR Marker
• Tamil Nadu [Vigna unguiculata (L ) Walp ]
• Cowpea varieties of Tamil Nadu
• SSR marker based DNA fingerprinting
• Genetic diversity
• Molecular marker
• Random amplified polymorphic DNA fingerprinting
• Pleurotus strains
• Genetic diversity characterization
• BMC Genomics
• Varietal adoption
• Cereal production
• Emphasized releases
• High yielding
• kĩ thuật RAPD
• kỹ thuật in dấu DNA
• phân tích DNA
• di truyền học
• DNA khuyếch đại
• SSR markers
• Andhra Pradesh
• Telangana with SSR markers
• Genetic similarity coefficient
• 19 standard reference rice varieties
• Simple sequence repeat DNA markers
• DNA sequence flanking
• Eukaryotic genomes
• BMC Bioinformatics
• Image analysis
• DNA patterns
• Generating dendrograms
• DNA barcode Orchids
• Molecular identification orchidaceae
• Molecular markers
• Molecular phylogeny
• Automated chip capillary electrophoresis
• Band matching
• Gel sample distortion
• Pattern recognition
• Microbial ecology
• Restriction fragment length polymorphism
• DNA sequencer
• Hybridization Analysis
• Chromosomal Deletions
• Microsatellite Analysis
• Mutation Analysis
• Human Cancer
• Ribosomal RNA analysis
• Glucoseyeast extract agar plates
• Genomic DNA
• Streptomyces violaceoruber TK21 rDNA
• Pathogenic nocardiae
• Rosa spp
• Pharmaceutical industries
• Unique species
• Bayesian analysis
• Commercial varieties
• Dianthus caryophyllus
• DNA markers
• Genetic relationship
• Spray and standard type carnations
• Granulation of cocoa powder incorporated
• Cocoa powder incorporated
• Green tea extract
• Fluid bed drier
• BMC Plant Biology
• Genetic purity
• Seed quality control
• Elaeis guineensis Jacq
• Molecular diversity
• Soybean genotypes
• Physiological characteristics
• Terminal restriction fragment polymorphism analysis
• Bacterial community structure
• DNA fingerprinting technique
• Ordination analyses
• Inherited Disorders
• DNA Fingerprints
• Biological Evidence
• Personal Identification
• Human Health
• Dichoropetalum alanyensis
• Mericarp features
• Scanning electron micrographs
• Mericarp surface sculpturing pattern