tailieunhanh - Determination of some fungal metabolite as influenced by temperature, time, pH and sugars by bioassay method
This study investigated the fungal biomass and inhibition of Bacillus megaterium DSM 32 with extracts of Fusarium equiseti, Penicillium italicum, Penicillium digitatum, Aspergillus candidus and Aspergillus parasiticus in modified Czapek Dox Broth. | Turk J Biol 25 (2001) 197-203 © TÜB‹TAK Determination of Some Fungal Metabolite as Influenced by Temperature, Time, pH and Sugars by Bioassay Method Metin DI⁄RAK Sütçü ‹mam University, Faculty of Arts and Science, Department of Microbiology, 46045, Kahramanmarafl-TURKEY Sami ÖZÇEL‹K Süleyman Demirel University, Faculty of Agriculture, Department of Microbiology, 32670, Atabey, Isparta-TURKEY Received: Abstract: This study investigated the fungal biomass and inhibition of Bacillus megaterium DSM 32 with extracts of Fusarium equiseti, Penicillium italicum, Penicillium digitatum, Aspergillus candidus and Aspergillus parasiticus in modified Czapek Dox Broth (mCDB) as affected by temperature (6, 15, 25 °C), incubation time (up to 4 wk), pH (, and ) and glucose the and fructose (, and ). The biomass production was greatest at 25 °C. In general, biomass production was greatest in media containing an addition of glucose or fructose; however, bacterial inhibition production was reduced by elevated sugars in mCDB over a 4-wk incubation period. Key Words: Fusarium sp., Aspergillus sp., Penicillium sp., Fungal metabolite. Farkl› S›cakl›k, Süre, pH ve fieker Varl›¤›nda Baz› Fungus Metabolitlerinin Biyolojik Ölçüm Metodu ile Belirlenmesi Özet: Bu çal›flmada, modifiye Czapek Dox buyyonda, Fusarium equiseti, Penicillium italicum, Penicillium digitatum, Aspergillus candidus ve Aspergillus parasiticus ekstraktlar›n›n farkl› s›cakl›k (6, 15, 25 °C), inkübasyon süresi (4 hafta), pH (, , ), glukoz ve fruktoz (% , , ) içeren ortamda Bacillus megaterium DSM 32’nin geliflmesinin engellenmesi ve biyomas oluflumu incelendi. Biyomas miktar›n›n 25 °C’de en fazla oldu¤u belirlendi. Genel olarak biyomas oluflumu % glukoz ve % fruktoz içeren ortamda daha fazla bulundu. Bakteri geliflmesinin engellenmesi, 4 hafta sonunda, fazla fleker ilave edilen mCDB’da genel olarak azald›¤› tespit edildi. Anahtar Sözcükler: Fusarium sp., .
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