tailieunhanh - Availability of use of total extracellular proteins in SDS page for typing Staphylococcus aureus and Coagulase negative staphylococci

A total of nineteen Staphylococcus references, nine of which are Staphylococcus aureus and ten coagulase-negative Staphylococci (CNS), were characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). | Turk J Biol 25 (2001) 145-151 © TÜB‹TAK Availability of Use of Total Extracellular Proteins in SDS-PAGE for Typing Staphylococcus aureus and Coagulase-Negative Staphylococci Sefa C. SAÇILIK Ankara University, Faculty of Sciences, Department of Biology, Tando¤an, 06100, Ankara-TURKEY Özlem OSMANA⁄AO⁄LU Gazi University, Faculty of Arts and Science, Department of Biology, Teknikokullar, 06500, Ankara-TURKEY A. Hakk› SAYAR Ankara University, Faculty of Sciences, Department of Biology, Tando¤an, 06100, Ankara-TURKEY Cumhur ÇÖKMÜfi Ankara University, Faculty of Sciences, Department of Biology, Tando¤an, 06100, Ankara-TURKEY Received: Abstract: A total of nineteen Staphylococcus references, nine of which are Staphylococcus aureus and ten coagulase-negative Staphylococci (CNS), were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Whole-cell protein (WCP) profiles obtained by SDS-PAGE were compared with banding patterns produced by SDS-PAGE of total extracellular proteins (TEP). No report has yet been issued which explains Staphylococcus typing by SDS-PAGE of extracellular proteins. SDS-PAGE of whole-cell extracts did not usefully distinguish different isolates of Staphylococci. Our preliminary results suggest that SDS-PAGE of total extracellular proteins provides additional criteria and characteristic banding patterns for the study of the epidemiology and characterization of Staphylococci. SDS-PAGE of extracellular proteins provided a rapid, reproducible and discriminative method for characterization of Staphylococci which is easy to perform and interpret. Key Words: Staphylococcus aureus, coagulase-negative staphylococci (CNS), protein profiles, typing. SDS-PAGE’de Hücre D›fl› Proteinlerinin Staphylococcus aureus ve Koagülaz Negatif Stafilokoklar›n Tiplendirimi ‹çin Kullan›m Uygunlu¤u Özet: Dokuz tanesi Staphylococcus aureus ve 10 tanesi koagülaz negatif stafilokok (CNS) olmak üzere toplam 19 adet referans .