tailieunhanh - Acute effects of trichloroacetic acid on serum enzyme levels and erythrocyte carbonic anhydrase activity in rats

The aim of the present study was to investigate the effects of a sublethal dose of trichloroacetic acid (TCA) on serum enzyme levels and erythrocyte carbonic anhydrase (CA) activity in rats under laboratory conditions. Ten Sprague-Dawley albino rats were divided in 2 groups of 5 rats. | Turk J Biol 30 (2006) 121-125 © TÜB‹TAK Acute Effects of Trichloroacetic acid on Serum Enzyme Levels and Erythrocyte Carbonic Anhydrase Activity in Rats 1 2 Halit DEM‹R , ‹smail ÇEL‹K 1 Department of Chemistry, Faculty of Arts and Sciences, Yüzüncü Y›l University, Van - TURKEY 2 Department of Biology, Faculty of Arts and Sciences, Yüzüncü Y›l University, Van - TURKEY Received: Abstract: The aim of the present study was to investigate the effects of a sublethal dose of trichloroacetic acid (TCA) on serum enzyme levels and erythrocyte carbonic anhydrase (CA) activity in rats under laboratory conditions. Ten Sprague-Dawley albino rats were divided in 2 groups of 5 rats. A 200 mg/kg dose of TCA was administered intraperitoneally to the 5 rats in the treatment group. An equal amount of saline solution was injected into the control group. Serum enzyme levels and erythrocyte CA activity of the rats were measured 1, 3, and 6 h after treatment. According to the results, while the TCA sublethal dose treatment caused significant increases in lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) after 1 and 3 h, aspartate aminotransferase (AST) significantly increased after 1, 3, and 6 h. No significant differences in the levels of alanine aminotransferase (ALT) and erythrocyte CA were observed in comparison to the controls. It was concluded that TCA may cause toxicity in rats. Key Words: Trichloroacetic acid, Serum enzymes, Erythrocyte Carbonic anhydrase, Rats Trikloroasetik asitin Akut Uygulamas›n›n S›çanlar›n Serum Enzim Düzeyleri ve Eritrosit Karbonik Anhidraz Aktivitesi Üzerine Etkileri Özet: Trikloroasetik asit (TCA) subletal dozda laboratuar flartlar›nda s›çanlar›n serum enzim düzeyleri ve eritrosit karbonik anhidraz (CA) aktivitesi üzerine etkileri araflt›r›ld›. 10 adet Sprague-Dawley albino iki gruba ayr›ld›. Her grup için befl adet s›çan kullan›ld›. Muamele grubuna 200 mg/ kg doz TCA periton alt›na (intraperitoneal) verildi. Kontrol grubuna