tailieunhanh - Analysis of the effects of chromatin modifying complexes on the transcription of retrotransposon Ty2-917 in Saccharomyces cerevisiae

Transcription of the yeast retrotransposon Ty2-917 is regulated by a complex set of regulatory regions located both upstream and downstream of the transcription initiation site. In this study, it was found that certain chromatin modifying factors are essential for the transcriptional regulation of Ty2-917. | Turk J Biol 30 (2006) 101-106 © TÜB‹TAK Analysis of the Effects of Chromatin Modifying Complexes on the Transcription of Retrotransposon Ty2-917 in Saccharomyces cerevisiae Sezai TÜRKEL, Bükay YEN‹CE Department of Biology, Faculty of Arts and Sciences, Uluda¤ University, 16059, Bursa - TURKEY Received: Abstract: Transcription of the yeast retrotransposon Ty2-917 is regulated by a complex set of regulatory regions located both upstream and downstream of the transcription initiation site. In this study, it was found that certain chromatin modifying factors are essential for the transcriptional regulation of Ty2-917. Among the chromatin binding factors tested in this research, non-histone proteins Nhp6A/B have the largest effect on the transcription of Ty2-917. In ∆nhp6A/B double mutant yeast strain transcription of Ty2 decreased 20-fold, indicating that these non-histone proteins are essential for the transcription of Ty2-917. Furthermore, it was also shown that the transcription of Ty2-917 decreased by 2-fold in the in ∆isw2 mutant, which shows that Isw2p is also involved in the activation of Ty2-917 transcription. In addition, results of this research show that the Spt7p, which is the critical subunit of SAGA complex, is essential for the maximal level transcription of Ty2-917. Key Words: Ty elements, Yeast, Retrotransposon, Chromatin factors, Transcription Kromatin Modifiye Eden Komplekslerin Saccharomyces cerevisiae Retrotranspozonu Ty2-917 Transkripsiyonuna Etkilerinin Analizi Özet: Maya retrotranspozonu Ty2-917’nin transkripsiyonu transkripsiyon bafllama yerinin alt ve üst bölgesinde yer alan kompleks yap›daki düzenleyici bölgeler taraf›ndan kontrol edilir. Bu çal›flmada baz› kromatin modifiye edici faktörlerin Ty2-917 transkripsiyonunun düzenlenmesi için gerekli oldu¤u bulundu. Bu araflt›rmada test edilen kromatin ba¤lanma faktörlerinden Ty2917 transkripsiyonuna en büyük etkisi olan faktör non-histon proteinleri olan Nhp6A/B olarak belirlendi. .