tailieunhanh - Colchicine induced tetraploidy and changes in allele frequencies in colchicine treated populations of diploids assessed with RAPD markers in Gossypium arboreum L.

Randomly Amplified Polymorphic DNA markers were used to evaluate diversity and similarities in populations treated with colchicine. Colchicine treatment generated differences beyond the levels of inter and intra-population variability. Contrary to expectations, colchicine-treated plants obtained by dipping in colchicine solution for 30 min did not contain more diversity than untreated populations. | Turk J Biol 30 (2006) 93-100 © TÜB‹TAK Colchicine-Induced Tetraploidy and Changes in Allele Frequencies in Colchicine-Treated Populations of Diploids Assessed with RAPD Markers in Gossypium arboreum L. Saeed RAUF*, Iftikhar Ahmad KHAN, Farooq Ahmad KHAN Department of Plant Breeding & Genetics, University of Agriculture, Faisalabad 38040-PAKISTAN Received: Abstract: Two cultivars of cotton G. arboreum L. cv. FDH-228 and HK-113 were treated with colchicine solution (1%) with 3 methods of application. Colchicine application at the seedling stage on shoot apex was successful. Tetraploid plants of arboreum (4x = 2n = 52) had larger vegetative, reproductive parts, but less frequent stomata and higher optical density of DNA than their normal diploid counterparts (2n = 26). Results also showed that individuals that escaped the duplicating effects of colchicine although treated, presented significant point mutagenic effects. Randomly Amplified Polymorphic DNA markers were used to evaluate diversity and similarities in populations treated with colchicine. Colchicine treatment generated differences beyond the levels of inter and intra-population variability. Contrary to expectations, colchicine-treated plants obtained by dipping in colchicine solution for 30 min did not contain more diversity than untreated populations. Key Words: Gossypium arboreum L., Colchicine, induced ploidy, Cultivars, Mutagenic, RAPD markers, Variability Introduction Colchicine (C22H25O6N), a product extracted from the seeds and bulbs of Colchicum autumnale L., is probably the most widely used chemical for induction of polyploidy. In the late 1930s Blakeslee and Avery (1) discovered that colchicines inhibit formation of spindle fibres and effectively arrest mitosis at the anaphase stage. At this phase the chromosome has multiplied but cell division has not yet taken place. Restriction of cell wall formation at this stage results in polyploid cells. These polyploid cells are larger than .

• Sinh học
• Gossypium arboreum L
• Induced ploidy
• RAPD markers
• Intra population variability
• Colchicine treatment generated