tailieunhanh - Lecture Microbiology - Chapter 18: Immune disorders

Lecture Microbiology - Chapter 18: Immune disorders. This chapter presents the following content: Immunological tests precipitin, immunodiffusion plates, agglutination test, neutralization test, complement fixation test, tagged antibody tests,. | IMMUNE DISORDERS CHAPTER 18 Copyright © 2012 John Wiley & Sons, Inc. All rights reserved. Immunological Tests Precipitin Precipitation – Soluble Ab (IgG or IgM) and Ag Immunodiffusion – Solid agar medium – Zones of precipitation still seen Copyright © 2012 John Wiley & Sons, Inc. All rights reserved. Immunodiffusion Plates Immunoelectrophoresis Copyright © 2012 John Wiley & Sons, Inc. All rights reserved. Agglutination Test Insoluble Ag plus Ab cause clumping – Easy to perform and read Direct agglutination – Clumps bacterial cells Indirect agglutination tests Place Ab or Ag on artificial particles – Latex spheres – Clay particles Red blood cell clumping = hemagglutination Copyright © 2012 John Wiley & Sons, Inc. All rights reserved. Neutralization Test Ab binds a toxin active site or viral binding site during incubation, neutralizing celldamaging effects The mix is placed over a cell layer in culture Absence of cytotoxic effects in culture indicates correct diagnosis Copyright © 2012 John Wiley & Sons, Inc. All rights .