tailieunhanh - Non histone proteins and the mediator complex are essential for the transcription of HXT2 and HXT4 genes in saccharomyces cerevisiae

Transcriptional activation of HXT2 and HXT4 genes require rapid reorganization of the chromatin structure on the promoter regions of these genes. In this study, it was found that the transcription of HXT2 and HXT4 genes decreased 4- to 7-fold in the yeast strain that does not contain the non-histone proteins 6A and 6B (Nhp6A/B). | Turk J Biol 30 (2006) 163-169 © TÜB‹TAK Non-Histone Proteins and the Mediator Complex are Essential for the Transcription of HXT2 and HXT4 Genes in Saccharomyces cerevisiae Sinem GÜZELVARDAR, Sezai TÜRKEL* Department of Biology, Faculty of Arts and Sciences, Uludag University, 16059 Bursa - TURKEY Received: Abstract: Transcriptional activation of HXT2 and HXT4 genes require rapid reorganization of the chromatin structure on the promoter regions of these genes. In this study, it was found that the transcription of HXT2 and HXT4 genes decreased 4- to 7-fold in the yeast strain that does not contain the non-histone proteins 6A and 6B (Nhp6A/B). In addition, results of this research indicated that Nhp6A/B are required for the rapid derepression and activation of HXT2 and HXT4 transcription upon glucose signaling. Furthermore, it was found that Med2p, which provides the connection between the transcriptional activators and the basal transcription factors, is also required for the activation of HXT2 and HXT4 transcription. Transcription of HXT2 and HXT4 decreased 2- to 13-fold in Med2 mutant S. cerevisiae cells. These results indicated that Nhp6A/B and the mediator complex are essential for the regulation of HXT2 and HXT4 gene transcription in S. cerevisiae. Key Words: Saccharomyces cerevisiae, glucose repression, hexose transporters, mediator, non-histone proteins Non-Histon Proteinler ve Mediator Kompleksi Saccharomyces cerevisiae’da HXT2 ve HXT4 Genleri Transkripsiyonu ‹çin Gereklidir Özet: HXT2 ve HXT4 genlerinin transkripsiyonunun aktivasyonu bu genlerin promotor bölgelerindeki kromatin yap›s›n›n h›zl› bir flekilde yeniden organizasyonunu gerektirir. Bu çal›flmada non-histon proteinler olan 6A ve 6B (Nhp6A/B) içermeyen maya suflunda HXT2 ve HXT4 genleri transkripsiyonunun 4-7 kat azald›¤› bulundu. Buna ek olarak bu çal›flman›n sonuçlar› Nhp6A/B’nin glukoz sinyalinin al›nmas›yla HXT2 ve HXT4 genleri transkripsiyonunun h›zl› bir flekilde aktivasyonu .

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