tailieunhanh - Đặc tính sinh học của chủng vius PRRS KTY - 06 phân lập tại Việt Nam và đánh giá đáp ứng miễn dịch của lợn khi tiêm hỗn hợp dịch kháng nguyên virus vô hoạt

Bài viết Đặc tính sinh học của chủng vius PRRS KTY - 06 phân lập tại Việt Nam và đánh giá đáp ứng miễn dịch của lợn khi tiêm hỗn hợp dịch kháng nguyên virus vô hoạt trình bày: Kết quả phân tích nguồn gốc phát sinh loài dựa trên trình tự gên ORRS của chủng KTY-06 đã chỉ ra chủng virus này thuộc genotype Bắc Mỹ,. . | Vietnam J. Agri. Sci. 2016, Vol. 14, No. 10:1631-1638 Tgp ch( KH Nong nghi$p Vi^t Nam 2016, tgp 14, s6 10:1631-1638 CHARACTERIZATION OF KTY-06 FIELD PRRS STRAIN ISOLATED IN VIETNAM AND EVALUATION OF ANTIBODY PRODUCING OF THE INACTIVATED VIRUS IN PIG Nguyen Thi Lan^*, Trinh Dinh Thau\ Yamaguchi RyojiS Nguyen Van Giap\ Luong Quoc Hung\ Nguyen Thi Yen', Nguyen Huu Nani\ Nguyen Thi Hoa', Le Huynh Thanh Phuong' ' Faculty of Veterinary Medicine, Vietnam National University of Agriculture ^Faculty of Agriculture, University ofMiyazaM, Japan Email*: nguyenlan@ Received dated: Accepted date: ABSTRACT KTY-06 virus strain was isolated from 2-week-old piglet in a PRRS outbreak in Northern Vietnam in 2015 that had clinical signs, gross findings, and microsopic lesions specific with HP-PRRS. Results of a phylogenetic analysis of the open reading frame 5 region of the KTY-06 strain showed it to be a North American genotype and classified into sublineage , in which highly pathogenic Chinese PRRSV strains are also clustered. Passaging in Marc-145 cells, KTY-06 gradually induced 35% up to 100% CPE between 36 to 60 hours post inoculation. The titer of KTY-06 was X 10^ TClDso/25pl. Of which, titer of the free viaises in the supernatant was higher than the titer of the cell associated virusese. Formalin-inactivated KTY-06 was able to stimulate pigs to produce specific antibodies against PRRSV. The immune response was detectable 14 day post immunization (dpi) (S/P ratio was ± ), peaked at 42 dpi (S/P ratio was ±, ), and then declined at 49 dpi. The results suggest that KTY-06 was a candidate for strain selection for producing PRRS vaccine. Keyvirards; Biology, antigenicity, PRRSV, KTY-06, Vietnam. Dac tinh sinh hoc cua chOng virus PRRS KTY-06 phan lap t^i Viet Nam va danh gia dap ifng mien dich cua Icrn l(hi tiem hon dich Ichang nguyen virus vo hoat T6M TAT ChOng virus KTY-06 duoc ph^n lap lit Ion con .