tailieunhanh - Chitinase production by Serratia marcescens GG5

Swollen chitin, flake chitin, powder chitin, and mushroom paste were used as substrates for chitinase production by Serratia marcescens GG5 in submerged fermentation. Enzyme production was U/ml when the organism was grown in M9 medium supplemented with swollen chitin and soluble starch. Scanning electron microscopy revealed that Serratia marcescens GG5 digested the chitin flakes by producing chitinase. | Turk J Biol 32 (2008) 231-236 © TÜBİTAK Chitinase Production by Serratia marcescens GG5 Gursharan SINGH, Joginder Ram SHARMA, Gurinder Singh HOONDAL Department of Microbiology, Panjab University, Chandigarh - INDIA Received: Abstract: Swollen chitin, flake chitin, powder chitin, and mushroom paste were used as substrates for chitinase production by Serratia marcescens GG5 in submerged fermentation. Enzyme production was U/ml when the organism was grown in M9 medium supplemented with swollen chitin and soluble starch. Scanning electron microscopy revealed that Serratia marcescens GG5 digested the chitin flakes by producing chitinase. Key Words: Swollen chitin, Serratia marcescens, chitinase, production Serratia marcescens GG5 ile Kitinaz Üretimi Özet: Son yıllarda yapılan çalışmalarda, sıvı ortam fermentasyonunda Serratia marcescens GG5 tarafından kitinaz üretimi için substrat olarak swollen kitin, tanecikli kitin, toz kitin ve mantar ezmesi kullanılmıştır. % 0,5 swollen kitin ve % 0,5 çözülebilir nişasta ilave edilmiş M9 ortamında organizma büyürken enzim üretimi 0,3 U/ml’dir. Taramalı elektron mikroskobu (SEM), Serratia marcescens GG5’in kitinaz üretimi sırasında kitin taneciklerini parçaladığını ortaya çıkarmıştır. Anahtar Sözcükler: Swollen kitin, Serratia marcescens, kitinaz, üretim Introduction Chitin is a polysaccharide composed of -1,4 N-acetyl D-glucosamine. It is highly distributed in nature, as a constituent of insect exoskeleton, shells of crustaceans, 11 and fungal cell walls. More than 1 × 10 t is estimated to be produced annually in marine waters alone, mostly by copepods (1). Chitinases (. ) are enzymes capable of hydrolyzing insoluble chitin to its oligo and monomeric components. Chitinases are present in bacteria and plants with a diversity of roles such as chitin metabolism in growing hyphae, defense mechanisms in response to pathogens, abiotic stress, and in nutrition and parasitism. In addition, .