tailieunhanh - Barley germplasms developed for scald disease resistance exhibited a high level of genetic diversity based on SRAP markers

The objective of this study was to assess the genetic diversity and genetic relationships among 59 Turkish barley (Hordeum vulgare L.) germplasm lines (maintained for scald disease resistance breeding) using sequence-related amplified polymorphism (SRAP) markers. | Turk J Biol 34 (2010) 271-279 © TÜBİTAK doi: Barley germplasms developed for scald disease resistance exhibited a high level of genetic diversity based on SRAP markers Ayten DİZKIRICI1, Zeki KAYA1,*, H. Elif GÜREN1, Hikmet BUDAK2 1 Department of Biological Sciences, Middle East Technical University, 06531 Ankara - TURKEY 2Department of Biological Sciences and Bioengineering, Sabancı University, 34956 İstanbul - TURKEY Received: Abstract: The objective of this study was to assess the genetic diversity and genetic relationships among 59 Turkish barley (Hordeum vulgare L.) germplasm lines (maintained for scald disease resistance breeding) using sequence-related amplified polymorphism (SRAP) markers. Seventeen SRAP primer combinations produced 83 polymorphic markers, with a mean polymorphism of . The dendrogram created based on Nei’s unweighted-pair group method using arithmetic average (UPGMA) indicated that there were 4 main clusters, which was supported by principle component analysis (PCA). Cluster I primarily included only scald-resistant germplasm lines, while clusters III and IV consisted of only scaldsensitive lines, but cluster II had both scald-resistant and scald-sensitive barley germplasm lines. The coefficients of genetic similarity among the genotypes ranged from to , with a cophenetic correlation (r = ) suggesting that the cluster analysis moderately represented the similarity matrix. The results indicate that a large amount of the genetic diversity present could be of great use in the development of future scald-resistant barley lines. Key words: Barley, scald disease, SRAP, genetic diversity SRAP belirteçlerinin yaprak lekesi hastalığına dirençlilik için geliştirilmiş arpa gen kaynaklarında ortaya çıkardığı yüksek genetik çeşitlilik Özet: Bu çalışmanın amacı, yaprak lekesi hastalığına dayanıklılık için geliştirilmiş elli dokuz Türk arpa (Hordeum vulgare L.) gen kaynağında genetik çeşitliliğin ve .