tailieunhanh - Molecular characterization of Rhizoctonia solani AG4 using PCR-RFLP of the rDNA-ITS region

Rhizoctonia solani isolates obtained from common beans (Phaseolus vulgaris L.) were included in an AG4 anastomosis group in accordance with hyphal anastomosis. In the subgrouping of AG4 isolates, PCR-RFLP patterns in the rDNA-ITS were used. | Turk J Biol 34 (2010) 261-269 © TÜBİTAK doi: Molecular characterization of Rhizoctonia solani AG4 using PCR-RFLP of the rDNA-ITS region Melike ÇEBİ KILIÇOĞLU, İbrahim ÖZKOÇ Department of Biology, Faculty of Science and Arts, Ondokuz Mayıs University, Samsun - TURKEY Received: Abstract: Rhizoctonia solani isolates obtained from common beans (Phaseolus vulgaris L.) were included in an AG4 anastomosis group in accordance with hyphal anastomosis. In the subgrouping of AG4 isolates, PCR-RFLP patterns in the rDNA-ITS were used. After obtaining the genomic DNA belonging to R. solani AG4, an approximately 700 bp amplification product of the region was obtained with PCR, using ITS1 and ITS4 universal primers. The PCR products were digested with MseI, HincII, AvaII, and MfeI restriction endonucleases, and different PCR-RFLP patterns were obtained for the AG4 HGI and AG4 HGII subgroups. In this study, in addition to the enzymes that were previously used in the AG4 subgrouping, AvaII restriction endonuclease was also seen to be effective in the subgrouping. In this way, the isolates that were grouped as R. solani AG4 according to anastomosis reactions were separated into 2 subgroups, HGI and HGII. This study indicates that the common beans in the Black Sea coastal region are more commonly infected by Rhizoctonia solani AG4 HGI. Key words: Rhizoctonia solani, rDNA-ITS, PCR-RFLP Rhizoctonia solani AG4 grubu fungusların rDNA-ITS bölgesinin PZR-RFLP ile moleküler karakterizasyonu Özet: Bu çalışmada, fasulye (Phaseolus vulgaris L.) bitkisinden elde edilen R. solani izolatları hifal anastomoz reaksiyonlarına göre AG4 anastomoz grubuna dahil edildi. AG4 izolatlarının altgruplarına ayrımında ise rDNA-ITS bölgesinin PZR-RFLP şablonlarından yararlanıldı. R. solani AG4 izolatlarına ait genomik DNA elde edildikten sonra, polimeraz zincir reaksiyonuyla (PZR) ITS-1 ve ITS-4 evrensel primerleri kullanılarak bölgesinin .

• Sinh học
• Rhizoctonia solani
• Beans Phaseolus vulgaris L
• Genomic DNA
• DNA homology
• rDNA ITS
• Sheath blight
• Rhizoctonia solani in vitro
• Rhizoctonia solani in soil
• Herbicides against sclerotial viability
• Different herbicides on the mycelial growth
• Mycelial growth of rhizoctonia solani
• Fungicides and Mungbean
• Rhizoctonia solani (Kuhn)
• Fungicides against Rhizoctonia solani
• Different bioagents
• Chemical measures
• Rhizoctonia solani Kuhn
• Trichoderma harzianum
• Pseudomonas fluorescence
• Chilli (Capsicum annum L )
• Rhizoctonia solani Kuhn in vivo
• Root rot
• Botanicals against rhizoctonia solani
• Root rot in soybean
• In vitro evaluation
• Pseudomonas fluorescens
• Dual culture
• In vitro and in soil
• Rhizoctonia solani in vitro and in soil
• Mycelial growth
• Web blight of soybean
• Sclerotial variability
• Rhizoctonia solani causal agent
• Molecular characterization
• Causing sheath blight in rice
• Silver nano
• Pathogen rhizoctonia solani kuhn
• Biogenic silver nanoparticles against rice
• Biocontrol agents
• Chickpea wet root
• Rot rhizoctonia solani
• Seed treatment with fungicides
• Bioagent on germination
• Capsicum annum
• Dry root rot
• Chilli (Capsicum annum)
• Antifungal nature
• Mycelial regeneration from sclerotia
• Blight of cowpea
• Sclerotial germination
• Cyamopsis tetragonoloba
• Root rot (Rhizoctonia solani)
• Clusterbean through fungicides
• Management of root rot
• Rootdip inoculation
• Stem application
• Tomato incited by Rhizoctonia solani
• Blight of tomato incited
• Sheath blight of rice
• Vitamins on epidemiological components
• Black scruf
• Disease severity
• Potato caused by Rhizoctonia solani
• Black scurf disease
• Trichoderma viride
• Rhizoctonia solani in organic gram
• Management of dry root rot caused
• Native Trichoderma for the management
• Management of wire stem
• Mustard caused by rhizoctonia solani
• Volatile compound produced
• Antagonistic potential of Trichoderma harzianum
• Sheath blight of maize
• Rhizoctonia solani causing banded leaf
• Host range
• Rice sheath blight fungus Rhizoctonia solani
• Host range studies
• Morphological characters
• Anastomosis grouping
• Group of Rhizoctonia solani
• Banded sheath blight
• Cross inoculation
• Rhizoctonia solani kuhn infecting small millets
• bài báo cáo thực tập
• bệnh thói củ do nấm
• vi khuẩn
• chủng nấm Rhizoctonia solani
• nấm Rhizoctonia solani
• Pathogenic variability
• Cultural characters
• Cotton collected
• Isolates of rhizoctonia solani
• Banded leaf and sheath blight
• Growth and development