tailieunhanh - Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus

Hexane, ethyl acetate, methanol, and aqueous extracts of Ranunculus marginatus d’Urv. var. trachycarpus (Fisch. & Mey.) Azn. and R. sprunerianus Boiss. were tested in vitro for their antioxidant and antibacterial activities. | Turk J Biol 34 (2010) 139-146 © TÜBİTAK doi: Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus Gülen İrem KAYA1,*, Nehir ÜNVER SOMER1, Sibel KONYALIOĞLU2, H. Tansel YALÇIN3, N. Ülkü KARABAY YAVAŞOĞLU3, Buket SARIKAYA1, Mustafa Ali ÖNÜR1 1Department of Pharmacognosy, Faculty of Pharmacy, Ege University, 35100 Bornova, İzmir - TURKEY 2Department of Biochemistry, Faculty of Pharmacy, Ege University, 35100 Bornova, İzmir - TURKEY 3Division of Biology, Faculty of Science, Ege University, 35100 Bornova, İzmir – TURKEY Received: Abstract: Hexane, ethyl acetate, methanol, and aqueous extracts of Ranunculus marginatus d’Urv. var. trachycarpus (Fisch. & Mey.) Azn. and R. sprunerianus Boiss. were tested in vitro for their antioxidant and antibacterial activities. Antioxidant activity of the extracts was determined by DPPH radical scavenging and Trolox equivalent antioxidant capacity assays. Methanol extracts showed the highest antioxidant activity in both assays. The total phenolics in the extracts were determined colorimetrically by using the Folin-Ciocalteau reagent. The total flavonoid content of the extracts was evaluated by a spectrophotometric method. The results obtained in the antioxidant activity tests were in positive correlation with the total phenolic and flavonoid contents of the extracts. An antibacterial activity analysis was carried out using paper disk diffusion and micro-well dilution techniques. All of the extracts displayed antibacterial activity against the tested bacteria in the disk diffusion method. The minimal inhibitory concentrations (MICs) of all the extracts of both Ranunculus species were found to be between 128 and 256 μg/mL. Key words: Antibacterial activity, antioxidant activity, flavonoid, phenol, Ranunculus marginatus var. trachycarpus, Ranunculus sprunerianus Ranunculus marginatus var. trachycarpus ve R. sprunerianus’un antioksidan ve antibakteriyal .