tailieunhanh - Determination of the relationship between some Centaurea species based on SDS-PAGE
The protein patterns of 47 Centaurea species from Turkey were obtained using SDS-PAGE methods. Relationships between Centaurea species and related genera were evaluated via use of a dendrogram. SDS-PAGE provided protein profiles for an important and applicable selection within Centaurea, particularly for the section Cheirolepis and its relatives. | Turk J Biol 34 (2010) 125-131 © TÜBİTAK doi: Determination of the relationship between some Centaurea species based on SDS-PAGE Tuna UYSAL, Emine ARSLAN, Osman TUGAY, Kuddisi ERTUĞRUL Department of Biology, Faculty of Science, Selçuk University, 42031 Konya - TURKEY Received: Abstract: The protein patterns of 47 Centaurea species from Turkey were obtained using SDS-PAGE methods. Relationships between Centaurea species and related genera were evaluated via use of a dendrogram. SDS-PAGE provided protein profiles for an important and applicable selection within Centaurea, particularly for the section Cheirolepis and its relatives. We determined that the technique could be used to resolve taxonomic and evolutionary problems in the genus Centaurea, as most of the Centaurea species studied exhibited special protein profiles and patterns. Key words: Centaurea, endemic, molecular analysis, SDS-PAGE, taxonomy SDS-PAGE metodu kullanılarak bazı Centaurea türleri arasındaki akrabalıkların belirlenmesi Özet: Bu çalışmada Türkiye’den 47 Centaurea türüne ait protein örnekleri SDS-PAGE metodu ile elde edildi. Centaurea türleri ve ilgili cinsler arasındaki akrabalıklar bir dendrogram kullanılarak değerlendirildi. SDS-PAGE Centaurea içinde uygulanabilir ve önemli bir ayırım için, özellikle Cheirolepis seksiyonu ve akrabalarıyla ilgili protein profilleri sağladı. Centaurea türlerinin büyük çoğunluğunun kendilerine özgü protein örneklerini ve profillerini sergilemeleri nedeniyle, cinsin taksonomik ve evrimsel proplemlerinin çözümünde bu tekniğin kullanılabileceğine karar verdik. Anahtar sözcükler: Centaurea, endemik, moleküler analiz, SDS-PAGE, taksonomi Introduction Turkey is renowned throughout the world for its floristic richness. It has numerous endemic and rare plants, most of which are known as locally growing species. In Turkey, Asteraceae is an important large family that includes some genera of superior quality, such as the genus Centaurea,
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