tailieunhanh - In vitro genotoxic effects of benzoyl peroxide in human peripheral lymphocytes

The aim of the present research was to investigate the genotoxic potency of benzoyl peroxide (BPO), a whitener of flour and cheese, using in vitro sister chromatid exchange (SCE), chromosome aberration (CA), and micronucleus (MN) tests in human peripheral lymphocytes. | Turk J Biol 34 (2010) 15-24 © TÜBİTAK doi: In vitro genotoxic effects of benzoyl peroxide in human peripheral lymphocytes Arzu YAVUZ1, Mehmet TOPAKTAŞ2,*, Erman Salih İSTİFLİ1 1 Department of Biology, Natural and Applied Science Institute, Çukurova University, Adana - TURKEY 2Faculty of Science and Letters, Department of Biology, Çukurova University, Adana - TURKEY Received: Abstract: The aim of the present research was to investigate the genotoxic potency of benzoyl peroxide (BPO), a whitener of flour and cheese, using in vitro sister chromatid exchange (SCE), chromosome aberration (CA), and micronucleus (MN) tests in human peripheral lymphocytes. Cells were exposed to 25, 50, 75, and 100 μg/mL concentrations of BPO for 24 and 48 h. BPO induced SCEs only at the highest concentration (100 μg/mL) for 48 h treatment. Furthermore, BPO induced CAs at 75 and 100 μg/mL concentrations for 24 h treatment, and at all concentrations for 48 h treatment. BPO induced CAs in a dose-dependent manner for 24 and 48 h treatment periods. Increased level of MN formation induced by BPO was only observed for the 48 h treatment period at the 2 highest concentrations (75 and 100 μg/mL). Statistically significant reductions in the proliferation index (PI) and mitotic index (MI) were only detected in cultures for the 48-h treatment period. Key words: Benzoyl peroxide, human lymphocytes, sister chromatid exchange, chromosome aberration, micronucleus İnsan periferal lenfositlerinde benzol peroksit’in in vitro genotoksik etkileri Özet: Bu çalışmanın amacı un ve peynir beyazlatmada kullanılan benzol peroksit (BPO)’in genotoksik etkisini insan periferal lenfositlerinde in vitro kardeş kromatid değişimi (KKD), kromozom aberasyonu (KA) ve mikronukleus (MN) testleriyle araştırmaktır. Hücreler 25, 50, 75 ve 100 μg/mL konsantrasyondaki BPO ile 24 ve 48 saat muamele edilmiştir. BPO KKD’yi sadece en yüksek konsantrasyonda (100 μg/mL) 48 saatlik muamelede uyarmıştır.