tailieunhanh - Quantification of 2,3,5,4’- tetrahydroxystilbene-2-O-β-D-Glucoside in dry extract of Radix fallopiae multiflorae by HPLC

Objectives: Establishing an HPLC method that is suited with current conditions of the laboratory to quantify 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside (THSG) in dry extract of Radix Fallopiae multiflorae. | Journal of military pharmaco-medicine No7-2016 QUANTIFICATION OF 2,3,5,4’TETRAHYDROXYSTILBENE-2-O-β-D-GLUCOSIDE IN DRY EXTRACT OF Radix Fallopiae multiflorae BY HPLC Hoang Viet Dung*; Nguyen Van Bach*; Pham Duc Thinh* SUMMARY Objectives: Establishing an HPLC method that is suited with current conditions of the laboratory to quantify 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside (THSG) in dry extract of Radix Fallopiae multiflorae. Material and methods: Parameters of the method are validated including: linear range, precision, accuracy. Results: Chromatographic conditions of the method: using Sunfire RP-C18 column, acetonitrile and aqueous formic acid as mobile phase following a multistep gradient program, monitored at 320 nm. The established method has good precision (RSD = ) and accuracy (recovery ratio = - , average = , RSD = ). Conclusion: HPLC method for quantifying THSG in dry extract of Radix Fallopiae multiflorae has been developed and validated. * Key words: Fallopia multiflora; 2,3,5,4’-tetrahydroxystilben-2-O-β-D-glucosid; HPLC method. INTRODUCTION Fallopia multiflora (Thunb.) Haralds. was traditionally used to treat several diseases including: attenuated body, white-haired, good for blood. [4]. Actually, this medicinal plant is the major component in some modern products that are more easily used. Following this trend, we are studying on preparing dry extract of Radix Fallopiae multiflorae as a raw material to prepare some products such as capsule and powder drug. THSG is a compound of stilbenoid group that was seen in Radix Fallopiae multiflorae. This compound possesses some important bioactivities as reducing blood lipid, anti-oxidant, antiinflammation So that, THSG was selected as a bioactive marker for quality control of this dry extract as well as end-products afterwards. This research is carried out to establish HPLC method for determining THSG in dry extract of Radix Fallopiae multiflorae that is .