tailieunhanh - Quantitative study for determination of astilbin in smilax glabra by high performance liquid chromatography

Objectives: By using high performance liquid chromatography (HPLC), develop and validate an analytical method for astilbin. Methods: Development of chromatographic conditions for astilbin quantification in Rhizoma Smilacis glabra. The method was validated according to ICH guidelines. | JOURNAL OF MILITARY PHARMACO-MEDICINE No7-2015 QUANTITATIVE STUDY FOR DETERMINATION OF ASTILBIN IN SMILAX GLABRA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY Le Viet Duc*; Vu Binh Duong**; Trinh Nam Trung**; Pham Thi Thanh Huong** SUMMARY Objectives: By using high performance liquid chromatography (HPLC), we develop and validate an analytical method for astilbin. Methods: Development of chromatographic conditions for astilbin quantification in Rhizoma Smilacis glabra. The method was validated according to ICH guidelines. Results: We investigated HPLC chromatographic conditions, including Phenomenex Gemini RP C18 column ( mm x 250 mm, 5 µm) at a flow rate of mL/min, detection wavelength of 291 nm, and an isocratic elution of aqueous acetic acid - acetonitrile (70:30, v/v). The method displayed an acceptable precision (RSD ≤ ) and accuracy. The detection and quantification limits were μg/ml and μg/ml respectively. Conclusion: The HPLC analytical method of astilbin was validated to meet requirements of ICH guidelines. * Key words: Smilax glabra; High performance liquid chromatography; Astilbin. INTRODUCTION Smilax glabra is a wild herb grown in the northern mountainous provinces of Vietnam. According to Vietnamese traditional medicine, Smilax glabra is often used in treatment of tendon diseases, worms, ulcers, diabetes, and detoxification. [1]. Concerning to compositions of Smilax glabra, astilbin, which is the main active constituent (figure 1) with high content, has been revealed to have hypouricemic, anti-inflammatory, analgesic anti-oxidation effects [2, 3]. Thus, quantitation for studies on extraction as well as quality control is very important. Figure 1: The chemical structure of astilbin. Vietnam Military Medical University has formulated several pharmaceutical products that contains Smilax glabra, such as: “Thong Phong Khang”, “Kien khop tieu thong”. * Minishy of Defense ** Vietnam Military Medical University Corresponding .