tailieunhanh - Quantitative analysis of silybin A and B in dry extract of milk thistle (Silybum marianum) by UPLC/UV-Vis method

Objectives: To establish an UPLC/UV-Vis method that is suited with current conditions of the laboratory to quantify silybin A and B in dry extract of milk thistle. Materials and methods: Parameters of the method are validated. | Journal of military pharmaco-medicine no7-2017 QUANTITATIVE ANALYSIS OF SILYBIN A AND B IN DRY EXTRACT OF MILK THISTLE (Silybum marianum) BY UPLC/UV-VIS METHOD Dang Truong Giang*; Vu Tuan Anh* Hoang Kim Vuong Nam**; Chu Van Men* SUMMARY Objectives: To establish an UPLC/UV-Vis method that is suited with current conditions of the laboratory to quantify silybin A and B in dry extract of milk thistle. Materials and methods: Parameters of the method are validated including: linear range, precision, accuracy. Results: Chromatographic conditions of the method: using Cortecs UPLC BEH column (C18; µm, x 50 mm), acetonitrile and aqueous formic acid as mobile phase following a multistep gradient program, monitored at 320 nm. The established method has good precision (RSD = ) and accuracy (recovery ratio = - , average = , RSD = ). Conclusions: UPLC/UV-Vis method for quantifying silybin A and B in dry extract of milk thistle has been developed and validated. * Keywords: Milk thistle; Silybin A, B; HPLC. INTRODUCTION Extract from the milk thistle seeds (Silybum marianum) is being used as a herbal therapy for hepatotoxicity and acute and chronic liver diseases [1, 2]. The pharmacological active ingredient is the flavonoid complex called silymarin, the main constituent accounts for about 80% of the extract [3]. Silymarin is a complex of at least seven flavonolignans that are the most common class of compounds present in milk thistle extract and one flavonoid, taxifolin. The relative abundance of each compound may vary depending on the sources of botanical materials, suppliers and extraction processes. Silybin represents about 50% to 70% of the silymarin extract. Silybin (figure 1), a stabilizer of liver cell membrane, has the effect of protecting the membrane of liver cells and enhancing its function and also protect the liver function from deterioration resulting from the invasion by deleterious substances [4]. Thus, silybin is .

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