tailieunhanh - Study on protective effect of specific igy antibody on cholera toxin intoxicated suckling mice

Objectives: To evaluate the protective effect of anti-choleratoxin IgY antibody on choleratoxin-intoxicated animal. Subjects and methods: Suckling mice (3-5 days old) were intoxicated with choleratoxin, then treated with anti-choleratoxin IgY. | Journal of military pharmaco-medicine no1-2018 STUDY ON PROTECTIVE EFFECT OF SPECIFIC IgY ANTIBODY ON CHOLERA TOXIN-INTOXICATED SUCKLING MICE Hoang Trung Kien*; Nguyen Anh Tuan* Le Thu Hong**; Nguyen Dang Dung* SUMMARY Objectives: To evaluate the protective effect of anti-choleratoxin IgY antibody on choleratoxin-intoxicated animal. Subjects and methods: Suckling mice (3 - 5 days old) were intoxicated with choleratoxin, then treated with anti-choleratoxin IgY. The protective effect of IgY antibody was evaluated by measuring survival time of choleratoxin-intoxicated suckling mice treated with anti-choleratoxin IgY and by histopathological analysis of epithelial cells of the mice's intestinal mucosa. Results: After 66 hours of choleratoxin infection, 65% of mice treated with anti-choleratoxin IgY were still alive (compared to 0% in control group); pathohistological images of the mice's intestinal mucosa showed less damages in those from mice treated with anti-choleratoxin IgY antibody compared to controls. Conclusions: IgY antibody against cholera toxin helps protect suckling mice from toxic effect of choleratoxin. * Keywords: Vibrio cholerae; IgY antibody; Sucking mice. INTRODUCTION Cholera is a serious diarrhea disease caused by gastrointestinal infection of Vibrio cholerae. In 2016, 172,454 cholera cases were reported by WHO, including 1,304 cases of deaths in 42 countries in the world with fatality rate of , and the total number of cholera cases and deaths has not decreased in the last five years, but the fatality rate for cholera is till high. Following the gastrointestinal infection, the bacteria secretes choleratoxin (CT), which consists of one A (active) and five B (binding) subunits. The B subunits of CT (CTB) can bind to GM1 ganglioside expressing on epithelial cells of the intestinal mucosa. Once bound, the cleavage between subunit A1 and A2 segments is facilitated and A1 portion moves into the cells. The A1 component stimulates the production of .