tailieunhanh - Proteomic profiling of multidrug-susceptible and resistant strains of Mycobacterium tuberculosis

In the present study, a combinantion of orbitrap mass spectrometry and nano-liquid chromatography was performed to identify the multdrug-resistant and susceptible M. tuberculosis proteomes. | All the annotated proteins in the TubercuList database [15] were subjected to the TMHMM program to predict the transmembrane helices in proteins. The results indicated that 807 proteins were predicted to transmembrane proteins and therefore 32% of TMH proteins have been identified in this study. The predicted TMH numbers of these proteins ranged from 1 to 15 (fig. 2b). Some important proteins with multiple TMHs were identified. Remarkably one of three transmembrane proteins with 15 TMHs was identified as probable peptidoglycan biosynthesis protein (MviN). In addition, 47 proteins were predicted by SignalP program to have a cleavable signal peptide for export across the cytoplasmic membrane. Lipoproteins are characterized by lipidated N-terminus and anchored in the membrane via a posttranslational modification [23]. Mycobacterial lipoproteins have been illustrated in forming a functionally diverse class of membrane-anchored or associated proteins [14, 24, 29]. Interestingly, we identified 41 out of the 99 predicted lipoproteins of

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