tailieunhanh - Cloning cdna sequence coding the KSA-1 lectin from red alga kappaphycus striatum cultivated in Vietnam

This cultivated alga may thus now contribute as a source of not only carrageenan, but also other bioactive compounds for biochemical and medicinal uses. KSA-1 lectin from this alga has been evaluated for biochemical properties, including high-mannose binding specificity, N-terminal amino acid sequence and molecular mass. In this study, we have cloned the cDNA clone encoding the lectin KSA-1. | Journal of Biotechnology 14(4): 689-697, 2016 CLONING cDNA SEQUENCE CODING THE KSA-1 LECTIN FROM RED ALGA KAPPAPHYCUS STRIATUM CULTIVATED IN VIETNAM Le Dinh Hung*, Dinh Thanh Trung Nha Trang Institute of Technology Research and Application, Vietnam Academy of Science and Technology * To whom correspondence should be addressed. E-mail: ledinhhungims@ Received: Accepted: SUMMARY The red alga Kappaphycus striatum is economically important food species and extensively cultivated in Vietnam. This cultivated alga may thus now contribute as a source of not only carrageenan, but also other bioactive compounds for biochemical and medicinal uses. KSA-1 lectin from this alga has been evaluated for biochemical properties, including high-mannose binding specificity, N-terminal amino acid sequence and molecular mass. In this study, we have cloned the cDNA clone encoding the lectin KSA-1. The full-length sequence of cDNA clone of KSA-1 lectin encoded a polypeptide of 268 amino acids including initiating methionine, with four tandemly repeated domains of about 67 amino acids, and sharing 43% sequence identity. The primary structure and the amino acid residues interact with mannopentaose core structure per a repeat domain of the KSA-1 lectin highly resemble and match those of anti-virus the lectin family in lower organinsms, including bacteria [BOA from Burkholderia oklahomensis EO147, MBHA from Myxococcus xanthus and PFA from Pseudomonas fluorescens Pf0-1], cyanobacteria [OAA from Oscillatoria agardhii] and red algae [ESA-2 from Eucheuma serra, EDA-2 from Eucheuma denticulatum, KAA-1 and KAA-2 from Kappaphycus alvarezii and KSA-2 from K. striatum]. Analysis of predicted secondary structure of KSA-1 showed twenty β-strands. Each repeated domain comprises the five β-strands and matches with number of βstrands of BOA lectin. Therefore, the red alga K. striatum could be a good source for application in biomedicine and biochemistry as materials for .

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• Journal of Biotechnology
• Cloning cdna sequence coding the KSA 1 lectin
• Red alga kappaphycus striatum cultivated in Vietnam
• Red alga kappaphycus striatum
• The cDNA clone
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