tailieunhanh - Electrophoretic aspects of blood serum proteins of the genus Apodemus in the Black Sea region

Globulin and albumin proteins of Apodemus populations in the Black Sea region were examined using SDS-PAGE (sodium dodecyl sulphate - polyacrylamide gel electrophoresis). There were 7-11 bands in the globulin region and 1-3 bands in the prealbumin zone. It was determined that there were variations in both the globulin region and the prealbumin zone in populations of this genus. | Turk J Biol 26 (2002) 125-131 © TÜB‹TAK Electrophoretic Aspects of Blood Serum Proteins of the Genus Apodemus in the Black Sea Region Reyhan ÇOLAK Ankara University, Faculty of Science, Department of Biology, 06100 Beflevler, Ankara - TURKEY Received: Abstract: Globulin and albumin proteins of Apodemus populations in the Black Sea region were examined using SDS-PAGE (sodium dodecyl sulphate - polyacrylamide gel electrophoresis). There were 7-11 bands in the globulin region and 1-3 bands in the prealbumin zone. It was determined that there were variations in both the globulin region and the prealbumin zone in populations of this genus. A band was observed in the postalbumin zone with different electrophoretic mobilities including slow and fast. This different mobility in the postalbumin zone separated Apodemus specimens into two different populations in sympatry. These findings showed that at least two populations of the genus Apodemus live in the Black Sea region instead of one. Key Words: Apodemus, albumin, globulin, SDS-PAGE, Black Sea region Karadeniz Bölgesi’ndeki Apodemus Cinsi (Mammalia:Rodentia)’nin Kan Serum Proteinlerinin Elektroforetik Özellikleri Özet: Bu çal›flmada, Karadeniz Bölgesindeki Apodemus populasyonlar›n›n albumin ve globulin proteinleri SDS-PAGE (sodium dodecyl sulphate - polyacrylamide gel electrophoresis) tekni¤ine dayanarak incelendi. Globulin bölgesinde 7-11 bant ve prealbumin zonunda ise 1-3 bant gözlendi. Apodemus cinsi populasyonlar›nda hem globulin bölgesinde hem de prealbumin zonunda varyasyonlar belirlendi. Postalbumin zonunda yavafl ve h›zl› oImak üzere farkl› elektroforetik mobiliteye sahip tek bir bant›n varl›¤› ortaya ç›kar›ld›. Postalbumin zonundaki bu farkl› mobilite Apodemus örneklerini simpatrik olarak yaflayan iki farkl› populasyona ay›rd›. Elde edilen bulgular Karadeniz bölgesinde Apodemus cinsine ait bir populasyonun de¤il iki populasyonun yaflad›¤›n› ortaya koydu. Anahtar Sözcükler: Apodemus, albumin, .

• Sinh học
• Apodemus populations
• Sodium dodecyl sulphate
• Polyacrylamide gel electrophoresis
• Serum proteins
• Biogeographical aspects
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