tailieunhanh - Properties of the rhodopseudomonas palustris strains isolated from an Alkaline lake in Turkey

31 photoheterotrophic bacteria were isolated from Lake Akşehir sediment. Identification of the strains was based on morphological properties, motility and pigment composition, and also physiological properties such as carbon utilization and ability to respire anaerobically and aerobically in the dark. | Tr. J. of Biology 23 (1999) 457–463 © TÜBİTAK Properties of the Rhodopseudomonas palustris Strains Isolated From an Alkaline Lake in Turkey Gönül ÇETİNKAYA DÖNMEZ Department of Biology, Faculty of Science, University of Ankara, Tandoğan, Ankara–TURKEY Ayten ÖZTÜRK Department of Biology, Faculty of Ed. Science, University of Niğde, Niğde–TURKEY Lütfü ÇAKMAKÇI Department of Food Engineering, Faculty of Agriculture, University of Ankara, Ankara–TURKEY Received: Abstract: 31 photoheterotrophic bacteria were isolated from Lake Akşehir sediment. Identification of the strains was based on morphological properties, motility and pigment composition, and also physiological properties such as carbon utilization and ability to respire anaerobically and aerobically in the dark. All strains were identified as Rhodopseudomonas palustris, but some strains were distinct from R. palustris with regard to vitamin requirement, malate utilization and aerobic growth in the dark. Until now, R. palustris had not been reported from an alkaline lake, and the strains growing alkaline pH (9) could be new strains of R. palustris. Key Words: Rhodopseudomonas palustris, isolation, identification, Alkaline Lake. Türkiye’deki Bir Alkali Gölden İzole Edilen Rhodopseudomonas palustris Suşlarının Özellikleri Özet: Akşehir gölünden fotoheterotrofik bakteriler izole edilmiştir. Elde edilen 31 izolat, morfolojik özellikleri, hareketlilikleri, oluşturdukları pigmentleri, karbon kaynağı olarak kullandıkları substratlar ve karanlıkta aerobik gelişme özelliklerine göre identifiye edildiğinde, bu suşların Rhodopseudomonas palustris türüne ait olduğu belirlenmiştir. Ancak izole edilen bazı suşların, vitamin ihtiyacı, mannitol kullanımı ve karanlıkta aerobik gelişme yönünden R. palustris’den farklı özellikler gösterdiği tespit edilmiştir. Bu konuda zamanımıza kadar yapılan çalışmalarda, R. palustris şuşlarının alkali göllerden izole edilmediği görülmüştür. Alkali koşullarda (pH: 9) gelişen bu .