tailieunhanh - Determination of the presence of crp genes in Staphylococcus aureus, Staphylococcus epidermidis, Lactobacillus delbrueckii and Corynebacterium verasus

Polymerase chain reaction (PCR) was employed to detect the presence of cyclic AMP receptor protein (CPR) in a number of diverse organisms. In PCR, two primers specific to the crp gene of Escherichia coli were used. | Tr. J. of Biology 23 (1999) 445–450 © TÜBİTAK Determination of the Presence of crp genes in Staphylococcus aureus, Staphylococcus epidermidis, Lactobacillus delbrueckii and Corynebacterium verasus Ali Osman BELDÜZ, Zihni DEMİRBAĞ, Sabriye DÜLGER Karadeniz Teknik Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, 61080, Trabzon–TURKEY Ali Osman KILIÇ Karadeniz Teknik Üniversitesi, Tıp Fakültesi, 61080, Trabzon–TURKEY Received: Abstract:Polymerase chain reaction (PCR) was employed to detect the presence of cyclic AMP receptor protein (CPR) in a number of diverse organisms. In PCR, two primers specific to the crp gene of Escherichia coli were used. Staphylococcus aureus, Staphylococcus epidermidis, Lactobacillus delbrueckii and Corynebacterium verasus all showed the same size of PCR fragments (708 bp) and same restriction fragment length polymorphizm (RFLP). Key Words: crp, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Lactobacillus delbrueckii and Corynebacterium verasus. Staphylococcus aureus, Staphylococcus epidermidis, Lactobacillus delbrueckii ve Corynebacterium verasus’da crp Geninin Belirlenmesi Özet: Polimeraz zincir reaksiyonu (PCR), birkaç değişik organizmadaki siklik AMP reseptör proteininin (CRP) varlığının belirlenmesinde kullanıldı. PCR’nda Escherichia coli’nin crp geni için spesifik olan iki tane primer kullanıldı. Çalışmada, Staphylococcus aureus, Staphylococcus epidermidis, Lactobacillus delbrueckii ve Corynebacterium verasus’un benzer PCR fragmentlerine (708 bp) ve benzer restriksiyon fragmenti uzunluk polimorfizmi (RFLP)’ne sahip oldukları görüldü. Anahtar Sözcükler: crp, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Lactobacillus delbrueckii ve Corynebacterium verasus. Introduction The cyclic AMP receptor protein (CRP or CAP) is a positive regulator of gene expression. When complexed with cyclic AMP (cAMP), it binds specifically in the promotor region of the operons which it .