tailieunhanh - Decolorization of Orange II dye with the crude culture filtrate of white rot fungus, Coriolus versicolor

Decolorization of Orange II dye was demonstrated with crude culture filtrate of white rot fungus, Coriolus versicolor. It was determined that the rate and extent of decolorization were dependent on the age (growth phase) of the crude culture filtrate (CCF). | Tr. J. of Biology 22 (1998) 463-476 © TÜBİTAK Decolorization of Orange II Dye With the Crude Culture Filtrate of White rot Fungus, Coriolus versicolor Özfer YEŞİLADA İnönü University, Art and Science Faculty, Department of Biology, 44069 Malatya-TURKEY Birgül ÖZCAN Mustafa Kemal University, Art and Science Faculty, Department of Biology, Hatay-TURKEY Received: Abstract: Decolorization of Orange II dye was demonstrated with crude culture filtrate of white rot fungus, Coriolus versicolor. It was determined that the rate and extent of decolorization were dependent on the age (growth phase) of the crude culture filtrate (CCF). Dye decolorization abilities of the CCF of Funalia trogii, Pleurotus sajor-caju and Phanerochaete chrysosporium ME446, after incubation for 12 days, were also studied. Phanerochaete chrysosporium ME446 was observed to be unable to decolorize any of 4 dyes used. Cyanide and azide, which are known inhibitors of enzyme, inhibited dye decolorization ability of the CCF. Heat treatment terminated decolorization activity of CCF. The decolorization activity determined in this study was manganase independent activity and veratryl alcohol had no influence on it. Results showed that both H2O2 dependent and independent enzymes could play a role in the reduction of the color of Orange II dye. Moreover, this is the first study which correlates the age of culture filtrate with decolorization activity. Key Words: Orange II, Dye, Decolorization, White rot fungi, Coriolus versicolor Ham Kültür Filtratı Kullanarak Orange II Boyasının Renginin Giderimi Özet: Bu çalışmada beyaz çürükçül fungus olan Coriolus versicolor’un kültür filtratının Orange II boyasının renk giderimi üzerine etkisi araştırılmıştır. Renk giderim hızı ve genişliğinin fungus ham kültür süzüntüsünün yaşına (üreme safhasına) bağlı olarak değiştiği saptanmıştır. 12 gün üretilmiş Funalia trogii, Pleurotus sajor-caju and Phanerochaete chrysosporium ME446’nın ham kültür filtratlarının da

• Sinh học
• Orange II
• White rot fungi
• Crude culture filtrate
• Funalia trogii
• Pleurotus sajor caju
• Phanerochaete chrysosporium ME446