tailieunhanh - In silico mining for alkaline enzymes from metagenomic dna data of gut microbes of the lower termite Coptotermes gestroi in Vietnam
In this study, using the Alcapred software, the metagenomic DNA sequences of the gut flora of Coptotermes gestroi were analyzed to identify the enzymes that were specifically adapted to alkaline condition. The results show that 737 of 943 ORFs (accounting for 72%) encoded proteases, 154 of 214 ORFs (holding 78%) encoded lipases and 338 of 575 ORFs (accounting for 59%) encoded cellulase and hemicelluase. | TAP CHI In silico SINHmining HOC 2016, for alkaline 38(3):enzymes 374-383 DOI: IN SILICO MINING FOR ALKALINE ENZYMES FROM METAGENOMIC DNA DATA OF GUT MICROBES OF THE LOWER TERMITE Coptotermes gestroi IN VIETNAM Nguyen Minh Giang1*, Do Thi Huyen2, Truong Nam Hai2 1 2 Ho Chi Minh University of Pedagogy Institute of Biotechnology, Vietnam Academy of Science and Techology ASBTRACT: The high alkaline proteases, lipases, cellulases and hemicellulases are important enzymes in research and industries. In this study, using the Alcapred software, the metagenomic DNA sequences of the gut flora of Coptotermes gestroi were analyzed to identify the enzymes that were specifically adapted to alkaline condition. The results show that 737 of 943 ORFs (accounting for 72%) encoded proteases, 154 of 214 ORFs (holding 78%) encoded lipases and 338 of 575 ORFs (accounting for 59%) encoded cellulase and hemicelluase. All those enzymes were predicted to be alkaline enzymes. This study provide an overview picture of the alkaline enzyme groups of the gut flora of C. gestroi, and provide a good database for mining, isolation of the genes to produce recombinant enzymes. Keywords: Coptotermes gestroi, alkaline enzyme, cellulase, gut, hemicellulase, lipase, metagenome, protease. Citation: Nguyen Minh Giang, Do Thi Huyen, Truong Nam Hai, 2016. In silico mining for alkaline enzymes from metagenomic dna data of gut microbes of the lower termite Coptotermes gestroi in Vietnam. Tap chi Sinh hoc, 38(3): 374-383. DOI: . *Corresponding author: gdthgiang@. INTRODUCTION Termites contribute substantially to the turnover of carbon and nitrogen in tropical ecosystems. Their diet consists exclusively of lignocellulose of various stages of decomposition, ranging from sound wood to humus. The digestion of this recalcitrant diet relies on the metabolic activities of a dense and diverse intestinal microbiota. In the gut of many lower termite
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