tailieunhanh - Identification of non plastidic adp glucose pyrophosphorylase unconventional partners in Arabidopsis thaliana

ADP-glucose pyrophosphorylase (ATP: alpha-glucose-1-phosphate adenylyl transferase, ADGase) previously has been studied as a key regulatory enzyme in the starch biosynthetic pathway in plant. | TAP CHI SINH HOC 2014, 36(4): 522-528 Identification of non plastidic adp-glucose pyrophosphorylase DOI: DOI: IDENTIFICATION OF NON PLASTIDIC ADP-GLUCOSE PYROPHOSPHORYLASE UNCONVENTIONAL PARTNERS IN Arabidopsis thaliana Pham Ngoc Vinh1*, Hong Gil Nam1, Nguyen Huy Hoang2 1 Pohang University of Science and Technology, South Korea, *phamngocvinh1988@ 2 Institute of Genome Research (IGR), Vietnam Academy of Science and Technology ABSTRACT: ADP-glucose pyrophosphorylase (ATP: alpha-glucose-1-phosphate adenylyl transferase, ADGase) previously has been studied as a key regulatory enzyme in the starch biosynthetic pathway in plant. Surprisingly, ADP-glucose pyrophosphorylase small subunit APS1 (ADG1) was found not only in chloroplast but also in non plastidic region, especially, small proportion in nucleus. To elucidate the novel mechanisms underlying non plastidic ADG1 actions, yeast two-hybrid screening was used to identify proteins associated with ADG1. Yeast two hybrid and co-immunoprecipitation (Co-IP) assay were used to confirm the interaction between ADG1 and interacting candidates. Furthermore, localization of interacting proteins was analyzed using Green Fluorescent Protein (GFP) fusion proteins under laser scanning microscopy. Two protein RPC4 (RNA polymerase III subunit) and LSU3 (Response to low Sulfur 3) were confirmed as strong candidates that interact with ADG1. Therefore, we hypothesized that non plastidic localized ADG1 might have additional function which mediates plant cellular metabolism status and intracellular signaling to regulate proper plant growth and development. Keywords: Arabidopsis thaliana, ADP-glucose pyrophosphorylase, co-immunoprecipiation, GFP, yeast two hybrid. INTRODUCTION ADP-glucose pyrophosphorylase (ATP: aglucose-1-phosphate adenylyl transferase, ADPG pyrophosphorylase, ADGase) is a key regulatory enzyme in the starch biosynthetic pathway in plants. This enzyme .