tailieunhanh - 3D culture and analysis of the expression of cancer stem cell markers from gastric cancer cell line MKN45
The result showed that only 6% single cancer cells developed into tumorspheres during culture process. Then, flow cytometry and immunofluorescence analysis was carried out to evaluate the expression of CSC markers CD44 and ALDH in samples taken from tumorspheres which arose from gastric CSCs. The results showed that gastric CSCs formed tumorspheres in 3D culture condition with a high expression of 92% and 40,1% for markers CD44 and ALDH, respectively. | JOURNAL OF MEDICAL RESEARCH 3D CULTURE AND ANALYSIS OF THE EXPRESSION OF CANCER STEM CELL MARKERS FROM GASTRIC CANCER CELL LINE MKN45 Ngo Thu Ha¹, Le Thi Thanh Huong¹, La Thi Huyen², Nguyen Phu Hung¹,³ ¹Thai Nguyen University of Sciences ²Institute of Biotechnology - Vietnam Academy of Science and Technology ³French National Institute of Health and Medical Research U1235, Nantes, France Gastric cancer is the fourth leading cause of worldwide cancer mortality, Vietnam belongs to the specified high-risk group of developing gastric cancer. Gastric cancer stem cells (CSCs) are considered to be the origin of gastric adenocarcinoma. 3D cell culture is an important step of identification of stem cell as well as CSC. In this study, a non-adherent cell culture model (3D) was developed with a culture medium that had growth factors selective for the development of gastric CSCs from gastric cancer cell line MKN45. The result showed that only 6% single cancer cells developed into tumorspheres during culture process. Then, flow cytometry and immunofluorescence analysis was carried out to evaluate the expression of CSC markers CD44 and ALDH in samples taken from tumorspheres which arose from gastric CSCs. The results showed that gastric CSCs formed tumorspheres in 3D culture condition with a high expression of 92% and 40,1% for markers CD44 and ALDH, respectively. Keywords: 3D culture, gastric cancer stem cell, CD44, ALDH, MKN45 I. INTRODUCTION Cancer is the leading cause of death globally. Gastric cancer has the fourth highest mortality, accounting for 723,000 deaths in 2012 according to World Health Organization [1]. Vietnamese are at high risk of developing gastric cancer. Important risk factors for development of gastric cancer include smoked foods, salted fish and meat, Corresponding author: Nguyen Phu Hung, Thai Nguyen University of Sciences Email: hungnguyenphu@ Received: 10 July 2017 Accepted: 09 Octorber 2017 JMR 111 E2 (2) - 2018 family history, chronic .
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