tailieunhanh - Enzymatic preparation of modulated– biodegradable hydrogel nanocomposites based chitosan/gelatin and biphasic calcium phosphate nanoparticles
In the study, injectable chitosan–4 hydroxyphenylacectamide acid (CHPA) and gelatin– tyramine (GTA)–based hydrogels were enzymatically prepared, in which could encapsulate biphasic calcium phosphate nanoparticles (BCP NPs) for enhancing bone regeneration. The in situ formation of hydrogel composite was varied from 35 to 80 seconds depending on concentration of H2O2. | Journal of Science and Technology 55 (1B) (2017) 185–192 ENZYMATIC PREPARATION OF MODULATED– BIODEGRADABLE HYDROGEL NANOCOMPOSITES BASED CHITOSAN/GELATIN AND BIPHASIC CALCIUM PHOSPHATE NANOPARTICLES Nguyen Tien Thinh1, Nguyen Thi Phuong2, Bui Thanh Thai2, Nguyen Trong Tri3, Nguyen Huynh Bach Son Long3, Tran Quoc Son3, Nguyen Tri Phu2, Nguyen Cuu Khoa2, Nguyen Dai Hai2, Tran Ngoc Quyen1, 2, * 1 School of Medicine and Pharmacy, TraVinh University 126 National Road 53, Ward 5, Tra Vinh City, Tra Vinh Province, Vietnam 2 Institute of Applied Materials Science, VAST 1 Mac Dinh Chi Street, Ben Nghe Ward, District 1, Ho Chi Minh City, Vietnam 3 Department of Chemical Engineering, Lac Hong University 10 Huynh Van Nghe Street, Buu Long Ward, Bien Hoa City, Dong Nai Province, Vietnam * Email: tnquyen979@ Received: 30 December 2016; Accepted for publication: 6 March 2017 ABSTRACT In the study, injectable chitosan–4 hydroxyphenylacectamide acid (CHPA) and gelatin– tyramine (GTA)–based hydrogels were enzymatically prepared, in which could encapsulate biphasic calcium phosphate nanoparticles (BCP NPs) for enhancing bone regeneration. The in situ formation of hydrogel composite was varied from 35 to 80 seconds depending on concentration of H2O2. Collagenase–mediated biodegradation of the hydrogel composite could be modulated from 3 days to over one month depending on amount of the formulated CHPA. Live/dead cell viability assay indicated that the hydrogel composite enhanced bone marrow mesenchymal stem cells (MSCs). The obtained results show a great potential of the hydrogel composites for bone regeneration due to its adjustable biodegradation, biocompatibility and enhancement in new bone formation. Keywords: chitosan, gelatin, horseradish peroxidase (HRP), hydrogel, collagenase. 1. INTRODUCTION Recently, biological hydrogels have played an important role in the advanced biomaterials for tissue regeneration and drug delivery systems. Several kinds of the injectable
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