tailieunhanh - Development of spectrophotometric method for determination of ceftazidime with the bratton– marshall reagent in pharmaceutical preparation

The reaction involves a threestep process of diazotization of the ceftazidime with mixture HCl and NaNO2 at 0–5 oC, to produce the corresponding diazonium salt, removal of residual nitrite with sulphamic acid and coupling with N-(1-naphthyl)ethylenediamine (NEDA),to give a purple colored product with λmax at 575 nm and stable for five hours. Beer’s law was obeyed at concentrations ranging from to µg/mL with correlation coefficient R2 = . | Journal of Science and Technology 55 (2) (2017) 220-228 DOI: DEVELOPMENT OF SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF CEFTAZIDIME WITH THE BRATTON– MARSHALL REAGENT IN PHARMACEUTICAL PREPARATION Nguyen Trung Dung*, Doan Thi Dao, Giap Dang Hoat, Nguyen Anh Son Faculty of Physics and Chemical Engineering, Le Quy Don Technical University, 236 Hoang Quoc Viet St, Cau Giay Dist, Ha Noi * Email: Nguyentrungdung1980@ Received: 18 May 2016; Accepted for publication: 1 February 2017 ABSTRACT A simple, rapid, sensitive and accurate spectrophotometric method for the determination of ceftazidime in pharmaceutical preparation has been developed. The reaction involves a threestep process of diazotization of the ceftazidime with mixture HCl and NaNO2 at 0–5 oC, to produce the corresponding diazonium salt, removal of residual nitrite with sulphamic acid and coupling with N-(1-naphthyl)ethylenediamine (NEDA),to give a purple colored product with λmax at 575 nm and stable for five hours. Beer’s law was obeyed at concentrations ranging from to µg/mL with correlation coefficient R2 = . The molar absorptivity, Sandell’s sensitivity, detection limit and quantitation limit of the method are × 104 , , and , of ceftazidime from auxiliary substances were - %. No interference was observed from various auxiliary substances in pharmaceutical formulations. The proposed method has been applied successfully to determine ceftazidime in pharmaceutical preparation as powder for injections (Biocetum Codzidime and Kabi). Keywords: spectrophotometry, ceftazidime, NEDA, Bratton-Marshall reagent, pharmaceutical preparation. 1. INTRODUCTION Cephalosporins remain important agents in the treatment of many types of bacterial infections because of their broad-spectrum activity, well-characterized pharmacokinetic and pharmacodynamic properties, and proven .