tailieunhanh - Phylogenetic diversity of microorganisms asscociated with three marine sponges from mien trung sea of Viet Nam

The phylogenetic analysis showed that bacterial community structures of the three investigated sponges similar to each other regarding 10 common phyla, although abundance of these phyla was different for each sponge. Phylum Thaumarchaeota was rich component for three sponges, especially in NT sponge ( %). | Journal of Science and Technology 55 (2) (2017) 168-177 DOI: PHYLOGENETIC DIVERSITY OF MICROORGANISMS ASSCOCIATED WITH THREE MARINE SPONGES FROM MIEN TRUNG SEA OF VIET NAM Nguyen Thi Kim Cuc1, * Ton That Huu Dat2, Tran Thi Hong2, Pham Viet Cuong1, 2 1 2 Institute of Marine Biochemistry, VAST, 18 Hoang Quoc Viet, Cau Giay, Ha Noi Mien Trung Institute for Scientific Research, VAST, 321 Huynh Thuc Khang, Hue * Email: kcnguyenthi@ Received: 27 October 2016; Accepted for publication: 24 November 2016 ABSTRACT Using culture - independent technique, hypervariable V4 region of 16S rDNA library sequencing by MiSeq, the baterial communities of three host sponges Rhabdastrellasp. DN, Spheciospongiasp. QT and Clathriasp. NT from Mien Trung sea were characterized. The phylogenetic analysis showed that bacterial community structures of the three investigated sponges similar to each other regarding 10 common phyla, although abundance of these phyla was different for each sponge. Phylum Thaumarchaeota was rich component for three sponges, especially in NT sponge ( %). In this sponge, 3 phyla Planctomycetes, Verrucomicrobia and Firmicutes were undetected in other 2 sponge samples. Phyla Cyanobacteria was observed only in DN sponge. The obtained amplicons were assigned in different taxonomic levels (class, order, family and genus) based on Silva database. At class level, Gammaproteobacteria was abundant in three sponges; and Caldilineae, Marine-group I were dominant in DN; mealwile, in QT other dominant classes were Marine-group I and Betaproteobacteria. For NT, they were Cytophaga and Deferribacteres. In general, all three sponges harbored abundant and genetically diverse microbial associated consortia and they shared several common bacterial operational taxonomic units, although with different abundance. Keywords: MiSeq sequencing, phylogenetic diversity, sponge associated microorganism, 16S rRNA, V4 region. 1. INTRODUCTION Microbes