tailieunhanh - Effect of sulfation on the structure and anticoagulant activity of ulvan extracted from green seaweed ulva reticulata

Ulvans are sulfated polysaccharides derived from the cell wall of green seaweeds. The chemical structure of ulvan extracted from Ulva reticulata is reported, focusing on investigation of the sulfated modification of the ulvan and the changes in structure and anticoagulant activity. The results showed that sulfated modification was able to change the ulvan conformational structure and markedly enhance its anticoagulant activity. | Journal of Science and Technology 54 (2C) (2016) 373-379 EFFECT OF SULFATION ON THE STRUCTURE AND ANTICOAGULANT ACTIVITY OF ULVAN EXTRACTED FROM GREEN SEAWEED ULVA RETICULATA Quach Thi Minh Thu1, Dang Vu Luong1, Nguyen Thi Nu2, Tran Thi Thanh Van3, Bui Minh Ly3, Thanh Thi Thu Thuy1, * 1 Institute of Chemistry, VAST, 18 Hoang Quoc Viet, Cau Giay, Hanoi 2 3 Hanoi Metropolitan University, 98 Duong Quang Ham, Hanoi Nhatrang Institute of Technology Research and Application, 2 Hung Vuong, Nha Trang city, Khanh Hoa * Email: thuyttt@ Received: 15 June 2016; Accepted for publication: 28 October 2016 ABSTRACT Ulvans are sulfated polysaccharides derived from the cell wall of green seaweeds. The chemical structure of ulvan extracted from Ulva reticulata is reported, focusing on investigation of the sulfated modification of the ulvan and the changes in structure and anticoagulant activity. The results showed that sulfated modification was able to change the ulvan conformational structure and markedly enhance its anticoagulant activity. Keywords: ulvan, green seaweed, Ulva reticulata, sulfation, structure, anticoagulant activity. 1. INTRODUCTION Ulvans are water-soluble sulfated polysaccharides derived from marine green seaweeds. They were reported to exhibit a wide range of physiological and biological activities such as anticancer, anticoagulant, antioxidant, antifungal and antitumor activities. Ulvan essentially contains rhamnose, xylose, glucuronic acid, iduronic acid and sulfate groups [1, 2, 3]. The chemical composition and structure of ulvan may vary depending on the algae species, place of cultivation and method of extraction [4, 5]. Green seaweed family has many species including Ulva lactuca, Ulva pertusa, Ulva rigida, U. rotundata, U. reticulata, Enteromorpha intestinalis, E. them, Ulva lactuca and Ulva reticulata are more popular [6]. In previous paper [7], we reported the results on extraction and structural determination of ulvan