tailieunhanh - In vitro antioxidant and antibacterial activities of fractionated extracts and isolation of bioactive components from breynia vitis idaea (Burm. f.) C. E. C. fischer leaves

This study investigated the antioxidant and antibacterial activities of ethyl acetate, nbutanol and ethanol extracts of Breynia vitis-idaea (.) C. E. C. Fischer leaves using in vitro assays;and isolated bioactive compounds from the fractioned extract which showed the best properties by column chromatography. | Journal of Science and Technology 54 (2C) (2016) 354-359 IN VITRO ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF FRACTIONATED EXTRACTS AND ISOLATION OF BIOACTIVE COMPONENTS FROM BREYNIA VITIS-IDAEA (BURM. F.) C. E. C. FISCHER LEAVES Nguyen Thi Lan Huong1, Nguyen Kim Minh Tam1, *, Huynh Ngoc Oanh1, Nguyen Thi Thu Huong2 1 Hochiminh city University of Technology, VNU – HCM, 268 Ly Thuong Kiet St, D10, HCM 2 Ginseng andMedicinal plants Research Centre, 41 Dinh Tien Hoang St, D1, HCM * Email: nguyenkmt@ Received: 15 June 2016 ; Accepted for publication: 24 October 2016 ABSTRACT This study investigated the antioxidant and antibacterial activities of ethyl acetate, nbutanol and ethanol extracts of Breynia vitis-idaea (.) C. E. C. Fischer leaves using in vitro assays;and isolated bioactive compounds from the fractioned extract which showed the best properties by column chromatography. All extracts showed significant radical scavenging activities and exhibited antibacterial activities against Enterococcus faecalis, Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA). Ethyl acetate extracts showed the highest free radical scavenging capacity with the IC50 values of and μg/ml (in DPPH and ABTS assays, respectively) and exhibited MIC values of , 1 and 1 mg/ml against the three bacteria, respectively. In addition, from ethyl acetate extracts, one pure compound has been obtained and identified as 6-O-benzoylarbutin. Keywords:Breynia vitis-idaea, antioxidant activities, antibacterial activities,6-O-benzoylarbutin. 1. INTRODUCTION Breynia vitis-idaea (.) Fischer (Euphorbiaceae) is an evergreen, large shrubwhich grows abundantly in Asian tropical regions, Africa, Australia and on some islands in the Pacific Ocean. In Vietnam, this plant has been discovered in many places, from northern regions down to Khanh Hoa, Lam Dong, Ba Ria – Vung Tau and Kien Giang (Phu Quoc island). Roots, leaves and barksof B. .