tailieunhanh - Optimization of the enzyme assisted extraction of essential oil from the leaves and branches of cinnamomum cassia using box wilson method

The process of enzyme assisted extraction of essential oil from the leaves and branches of the Vietnamese aromatic plant Cinnamomum cassia was studied and optimized using a Box-Wilson central composite design consisting of 05 independent variables (pH, temperature T, time τ, concentration of the enzyme Laccase, and concentration of the enzyme Cellic Htec2), and two dependent variables (reducing sugar and yield of essential oil). | Vietnam Journal of Science and Technology 55 (6) (2017) 690-697 DOI: OPTIMIZATION OF THE ENZYME ASSISTED EXTRACTION OF ESSENTIAL OIL FROM THE LEAVES AND BRANCHES OF CINNAMOMUM CASSIA USING BOX-WILSON METHOD Hoang Thi Bich1, 2, Le Mai Huong1, Nguyen Quyet Chien1, Dinh Thi Thu Thuy1, Le Tat Thanh1, Do Trung Sy3, Le Thi Thuy Hang4, Pham Hong Hai1 1 2 Institute of Natural Products Chemistry, VAST, 18 Hoang Quoc Viet, Ha Noi Graduate University of Science and Technology, VAST, 18 Hoang Quoc Viet, Ha Noi 3 Institute of Chemistry, VAST, 18 Hoang Quoc Viet, Ha Noi 4 Quality Assurance and Testing Center 1, Directorate for Standards, Metrology and Quality, 08 Hoang Quoc Viet, Ha Noi * Email: Received: 6 April 2017; Accepted for publication: 23 October 2017 Abstract. The process of enzyme assisted extraction of essential oil from the leaves and branches of the Vietnamese aromatic plant Cinnamomum cassia was studied and optimized using a Box-Wilson central composite design consisting of 05 independent variables (pH, temperature T, time τ, concentration of the enzyme Laccase, and concentration of the enzyme Cellic Htec2), and two dependent variables (reducing sugar and yield of essential oil). Secondorder polynomial equations were obtained for the responses, which fitted well with the experimental data. Optimal conditions for oil yield were found at pH = ; T = 44 oC; τ = 5h30'; Laccase = ml/g, and Cellic Htec2 = %. The experimental value ( % oil yield) was close to the predicted value ( %). The application of enzyme assisted extraction in combination with optimization using response surface methodology substantially improved the oil yield as compared with traditional method. Keywords: optimization, Box-Wilson central composite design, enzyme assisted extraction, essential oil, Cinnamomum cassia. 1. INTRODUCTION Cinnamomum cassia (L.) J. Presl (Vietnamese: Quế đơn, English: Chinese cassia .